Characterization of Limnospira platensis PCC 9108 R-M and CRISPR-Cas systems

The filamentous cyanobacterium Limnospira platensis, formerly known as Arthrospira platensis or spirulina, is one of the most commercially important species of microalgae. Due to its high nutritional value, pharmacological and industrial applications it is extensively cultivated on a large commercia...

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Autores: Castillo López, María, Guevara Acosta, Flor Govinda, Baldanta Callejo, Sara, Suárez Rodríguez, Patricia, Agudo, Lucía, Nogales, Juan, Díaz Carrasco, Asunción, Arribas-Aguilar, Fernando, Pérez-Pérez, Julián, García, Jose Luis, Galán, Beatriz, Navarro Llorens, Juana María
Formato: artículo
Fecha de publicación:2023
País:España
Recursos:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/92676
Acesso em linha:https://hdl.handle.net/20.500.14352/92676
Access Level:acceso abierto
Palavra-chave:CRISPR-Cas
Cyanobacteria
Limnospira platensis
Methyltransferase
Restriction-modification systems
Ciencias
24 Ciencias de la Vida
33 Ciencias Tecnológicas
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spelling Characterization of Limnospira platensis PCC 9108 R-M and CRISPR-Cas systemsCaracterizacion de Limnospira platensis PCC 9108 R-M y los sistemas CrispR-CasCastillo López, MaríaGuevara Acosta, Flor GovindaBaldanta Callejo, SaraSuárez Rodríguez, PatriciaAgudo, LucíaNogales, JuanDíaz Carrasco, AsunciónArribas-Aguilar, FernandoPérez-Pérez, JuliánGarcía, Jose LuisGalán, BeatrizNavarro Llorens, Juana MaríaCRISPR-CasCyanobacteriaLimnospira platensisMethyltransferaseRestriction-modification systemsCiencias24 Ciencias de la Vida33 Ciencias TecnológicasThe filamentous cyanobacterium Limnospira platensis, formerly known as Arthrospira platensis or spirulina, is one of the most commercially important species of microalgae. Due to its high nutritional value, pharmacological and industrial applications it is extensively cultivated on a large commercial scale. Despite its widespread use, its precise manipulation is still under development due to the lack of effective genetic protocols. Genetic transformation of Limnospira has been attempted but the methods reported have not been generally reproducible in other laboratories. Knowledge of the transformation defense mechanisms is essential for understanding its physiology and for broadening their applications. With the aim to understand more about the genetic defenses of L. platensis, in this work we have identified the restriction-modification and CRISPR-Cas systems and we have cloned and characterized thirteen methylases. In parallel, we have also characterized the methylome and orphan methyltransferases using genome-wide analysis of DNA methylation patterns and RNA-seq. The identification and characterization of these enzymes will be a valuable resource to know how this strain avoids being genetically manipulated and for further genomics studies.Universidad Complutense de Madrid20232023-01-0120232023-01-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/92676reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)InglésengComunidad de Madrid Not available S2018%2FBAA-4532Comunidad de Madrid Not available S2013%2FABI-2783Ministerio de Ciencia e Innovación Not available RTI2018-094399-A-I00Ministerio de Ciencia e Innovación Not available PID2019-108458RB-I00Ministerio de Ciencia e Innovación Not available TED2021–130689B-C33open accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/926762026-06-02T12:44:21Z
dc.title.none.fl_str_mv Characterization of Limnospira platensis PCC 9108 R-M and CRISPR-Cas systems
Caracterizacion de Limnospira platensis PCC 9108 R-M y los sistemas CrispR-Cas
title Characterization of Limnospira platensis PCC 9108 R-M and CRISPR-Cas systems
spellingShingle Characterization of Limnospira platensis PCC 9108 R-M and CRISPR-Cas systems
Castillo López, María
CRISPR-Cas
Cyanobacteria
Limnospira platensis
Methyltransferase
Restriction-modification systems
Ciencias
24 Ciencias de la Vida
33 Ciencias Tecnológicas
title_short Characterization of Limnospira platensis PCC 9108 R-M and CRISPR-Cas systems
title_full Characterization of Limnospira platensis PCC 9108 R-M and CRISPR-Cas systems
title_fullStr Characterization of Limnospira platensis PCC 9108 R-M and CRISPR-Cas systems
title_full_unstemmed Characterization of Limnospira platensis PCC 9108 R-M and CRISPR-Cas systems
title_sort Characterization of Limnospira platensis PCC 9108 R-M and CRISPR-Cas systems
dc.creator.none.fl_str_mv Castillo López, María
Guevara Acosta, Flor Govinda
Baldanta Callejo, Sara
Suárez Rodríguez, Patricia
Agudo, Lucía
Nogales, Juan
Díaz Carrasco, Asunción
Arribas-Aguilar, Fernando
Pérez-Pérez, Julián
García, Jose Luis
Galán, Beatriz
Navarro Llorens, Juana María
author Castillo López, María
author_facet Castillo López, María
Guevara Acosta, Flor Govinda
Baldanta Callejo, Sara
Suárez Rodríguez, Patricia
Agudo, Lucía
Nogales, Juan
Díaz Carrasco, Asunción
Arribas-Aguilar, Fernando
Pérez-Pérez, Julián
García, Jose Luis
Galán, Beatriz
Navarro Llorens, Juana María
author_role author
author2 Guevara Acosta, Flor Govinda
Baldanta Callejo, Sara
Suárez Rodríguez, Patricia
Agudo, Lucía
Nogales, Juan
Díaz Carrasco, Asunción
Arribas-Aguilar, Fernando
Pérez-Pérez, Julián
García, Jose Luis
Galán, Beatriz
Navarro Llorens, Juana María
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv CRISPR-Cas
Cyanobacteria
Limnospira platensis
Methyltransferase
Restriction-modification systems
Ciencias
24 Ciencias de la Vida
33 Ciencias Tecnológicas
topic CRISPR-Cas
Cyanobacteria
Limnospira platensis
Methyltransferase
Restriction-modification systems
Ciencias
24 Ciencias de la Vida
33 Ciencias Tecnológicas
description The filamentous cyanobacterium Limnospira platensis, formerly known as Arthrospira platensis or spirulina, is one of the most commercially important species of microalgae. Due to its high nutritional value, pharmacological and industrial applications it is extensively cultivated on a large commercial scale. Despite its widespread use, its precise manipulation is still under development due to the lack of effective genetic protocols. Genetic transformation of Limnospira has been attempted but the methods reported have not been generally reproducible in other laboratories. Knowledge of the transformation defense mechanisms is essential for understanding its physiology and for broadening their applications. With the aim to understand more about the genetic defenses of L. platensis, in this work we have identified the restriction-modification and CRISPR-Cas systems and we have cloned and characterized thirteen methylases. In parallel, we have also characterized the methylome and orphan methyltransferases using genome-wide analysis of DNA methylation patterns and RNA-seq. The identification and characterization of these enzymes will be a valuable resource to know how this strain avoids being genetically manipulated and for further genomics studies.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023-01-01
2023
2023-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/92676
url https://hdl.handle.net/20.500.14352/92676
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Comunidad de Madrid Not available S2018%2FBAA-4532
Comunidad de Madrid Not available S2013%2FABI-2783
Ministerio de Ciencia e Innovación Not available RTI2018-094399-A-I00
Ministerio de Ciencia e Innovación Not available PID2019-108458RB-I00
Ministerio de Ciencia e Innovación Not available TED2021–130689B-C33
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300724

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