Comparative genomic analysis of CRISPR/Cas systems in Thermococcus genomes
CRISPR/Cas systems are adaptive immune systems widespread in bacteria and archaea that confer protection against invading nucleic acids. However, beyond its role in prokaryotes, many applications have derived from these systems, especially in the genome engineering field. Such has been the impact of...
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| Tipo de recurso: | tesis de maestría |
| Fecha de publicación: | 2021 |
| País: | España |
| Institución: | Universitat Oberta de Catalunya (UOC) |
| Repositorio: | O2, repositorio institucional de la UOC |
| OAI Identifier: | oai:openaccess.uoc.edu:10609/126966 |
| Acceso en línea: | http://hdl.handle.net/10609/126966 |
| Access Level: | acceso abierto |
| Palabra clave: | CRISPR/Cas thermococcus structural features característiques estructurals características estructurales Bioinformatics -- TFM Bioinformàtica -- TFM Bioinformática -- TFM |
| Sumario: | CRISPR/Cas systems are adaptive immune systems widespread in bacteria and archaea that confer protection against invading nucleic acids. However, beyond its role in prokaryotes, many applications have derived from these systems, especially in the genome engineering field. Such has been the impact of CRISPR/Cas systems on this field, that they are considered a game-changing technology. This impact has triggered the interest in identifying and characterizing such systems in prokaryotes. Even so, the CRISPR/Cas systems of many prokaryotes, particularly in archaea, remain yet-to-be-explored. In this study, the CRISPR/Cas systems of Thermococcus are characterized through a comparative genomic analysis. Thermococcus is an archaeal genus that comprises thermophilic microorganisms. Thermococcus are of interest for the industry, mainly owing to their thermostable enzymes. The characterization of the systems identified in Thermococcus revealed their occurrence, diversity, organization and structural features. For said characterization, the use of online tools, specially created for CRISPR/Cas systems analyses, and software aimed at phylogenetic analyses have been required. Overall, a high occurrence and diversity of CRISPR/Cas systems have been detected in Thermococcus. The CRISPR loci, cas loci and structural features analyses also suggested likely horizontal gene transfer events among Thermococcus species. Furthermore, a pipeline to detect and characterize CRISPR/Cas systems in prokaryotic genomes has been generated, which can be employed for other species or genera. |
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