Implementation of oligonucleotide-gated supports for the electrochemical detection of Ochratoxin A

[EN] We report herein the design a hybrid material for the recognition of Ochratoxin A (OTA) by using an aptamer gated-material able to release electroactive species, which can be measured using differential pulse voltammetry. Mesoporous silica nanoparticles loaded with Methylene Blue and capped wit...

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Detalles Bibliográficos
Autores: Muthamizh, S., Ribes, Àngela, Anusuyajanakiraman, M., Narayanan, V., Soto Camino, Juan, Martínez-Máñez, Ramón|||0000-0001-5873-9674, Aznar, Elena|||0000-0003-0361-3876
Tipo de recurso: artículo
Fecha de publicación:2017
País:España
Institución:Universitat Politècnica de València (UPV)
Repositorio:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
Idioma:inglés
OAI Identifier:oai:riunet.upv.es:10251/102309
Acceso en línea:https://riunet.upv.es/handle/10251/102309
Access Level:acceso abierto
Palabra clave:Molecular gates
Mesoporous materials
Ochratoxin A
Sensing
Differential pulse voltammetry
Electron Microscopy Service of the UPV
QUIMICA INORGANICA
QUIMICA ANALITICA
Descripción
Sumario:[EN] We report herein the design a hybrid material for the recognition of Ochratoxin A (OTA) by using an aptamer gated-material able to release electroactive species, which can be measured using differential pulse voltammetry. Mesoporous silica nanoparticles loaded with Methylene Blue and capped with an OTA aptamer were prepared and characterised. A highly selective and sensitive response due to the displacement of the capping aptamer in the presence of OTA and subsequent MB release was found in aqueous media. Finally, the possible use of the gated material for the detection of OTA in a real matrix was also explored.