Design of oligonucleotide-capped mesoporous silica nanoparticles for the detection of miRNA-145 by duplex and triplex formation
[EN] The development of new strategies to detect microRNAs (miRNAS) has become an important challenge in thebiomedical ¿eld. We report herein the use of oligonucleotide-gated silica nanoparticles for the detection ofmiRNA-145. In the proposed design, mesoporous silica nanoparticles (MSNs) are loaded...
| Autores: | , , , , , , , |
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| Tipo de recurso: | artículo |
| Fecha de publicación: | 2018 |
| País: | España |
| Institución: | Universitat Politècnica de València (UPV) |
| Repositorio: | RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia |
| Idioma: | inglés |
| OAI Identifier: | oai:riunet.upv.es:10251/143135 |
| Acceso en línea: | https://riunet.upv.es/handle/10251/143135 |
| Access Level: | acceso abierto |
| Palabra clave: | Mesoporous silica materials Molecular gates MiRNA-145 Biosensor Triplex QUIMICA INORGANICA QUIMICA ANALITICA QUIMICA ORGANICA CIENCIA DE LOS MATERIALES E INGENIERIA METALURGICA |
| Sumario: | [EN] The development of new strategies to detect microRNAs (miRNAS) has become an important challenge in thebiomedical ¿eld. We report herein the use of oligonucleotide-gated silica nanoparticles for the detection ofmiRNA-145. In the proposed design, mesoporous silica nanoparticles (MSNs) are loaded with a ¿uorescentreporter (rhodamine B) and pores are blocked by speci¿c DNA oligonucleotides. The opening of the gated systemand dye delivery is selectively controlled by DNA-miRNA recognition. Moreover, the use of DNA capture probesable to form duplex or triplex structures between target miRNA and the complementary oligonucleotides hasbeen studied. By this simple procedure a limit of detection as low as 0.25 pM was found for miRNA. The methodwas successfully applied to detect miRNA-145 in serum samples, which demonstrates the high potential of thesecapped materials to detect miRNA for diagnostic purposes |
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