Functional Characterization of Four Olive Squalene Synthases with Respect to the Squalene Content of the Virgin Olive Oil

12 Páginas.-- 6 Figuras

Detalhes bibliográficos
Autores: Hernández, M. Luisa, Muñoz-Ocaña, Cristina, Posada, Pilar, Sicardo, M. Dolores, Hornero-Méndez, Dámaso, Gómez-Coca, R. B., Belaj, Angjelina, Moreda, Wenceslao, Martínez-Rivas, José Manuel
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/338413
Acesso em linha:http://hdl.handle.net/10261/338413
https://api.elsevier.com/content/abstract/scopus_id/85175218986
Access Level:acceso abierto
Palavra-chave:Olea europaea
SQS
Core collection
Gene expression
Olive fruit
Water stress
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spelling Functional Characterization of Four Olive Squalene Synthases with Respect to the Squalene Content of the Virgin Olive OilHernández, M. LuisaMuñoz-Ocaña, CristinaPosada, PilarSicardo, M. DoloresHornero-Méndez, DámasoGómez-Coca, R. B.Belaj, AngjelinaMoreda, WenceslaoMartínez-Rivas, José ManuelOlea europaeaSQSCore collectionGene expressionOlive fruitWater stress12 Páginas.-- 6 FigurasThe release of new olive cultivars with an increased squalene content in their virgin olive oil is considered an important target in olive breeding programs. In this work, the variability of the squalene content in a core collection of 36 olive cultivars was first studied, revealing two olive cultivars, 'Dokkar' and 'Klon-14', with extremely low and high squalene contents in their oils, respectively. Next, four cDNA sequences encoding squalene synthases (SQS) were cloned from olive. Sequence analysis and functional expression in bacteria confirmed that they encode squalene synthases. Transcriptional analysis in distinct olive tissues and cultivars indicated that expression levels of these four SQS genes are spatially and temporally regulated in a cultivar-dependent manner and pointed to OeSQS2 as the gene mainly involved in squalene biosynthesis in olive mesocarp and, therefore, in the olive oil. In addition, the biosynthesis of squalene appears to be transcriptionally regulated in water-stressed olive mesocarp.This work was supported by the Spanish Ministry of Science, Innovation, and Universities through research grant AGL2017-87871-R (AEI/FEDER, UE).Peer reviewedAmerican Chemical SocietyMinisterio de Ciencia, Innovación y Universidades (España)Agencia Estatal de Investigación (España)European CommissionConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202320232023info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/338413https://api.elsevier.com/content/abstract/scopus_id/85175218986reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-87871-RJournal of agricultural and food chemistryhttps://doi.org/10.1021/acs.jafc.3c05322Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3384132026-05-22T06:33:51Z
dc.title.none.fl_str_mv Functional Characterization of Four Olive Squalene Synthases with Respect to the Squalene Content of the Virgin Olive Oil
title Functional Characterization of Four Olive Squalene Synthases with Respect to the Squalene Content of the Virgin Olive Oil
spellingShingle Functional Characterization of Four Olive Squalene Synthases with Respect to the Squalene Content of the Virgin Olive Oil
Hernández, M. Luisa
Olea europaea
SQS
Core collection
Gene expression
Olive fruit
Water stress
title_short Functional Characterization of Four Olive Squalene Synthases with Respect to the Squalene Content of the Virgin Olive Oil
title_full Functional Characterization of Four Olive Squalene Synthases with Respect to the Squalene Content of the Virgin Olive Oil
title_fullStr Functional Characterization of Four Olive Squalene Synthases with Respect to the Squalene Content of the Virgin Olive Oil
title_full_unstemmed Functional Characterization of Four Olive Squalene Synthases with Respect to the Squalene Content of the Virgin Olive Oil
title_sort Functional Characterization of Four Olive Squalene Synthases with Respect to the Squalene Content of the Virgin Olive Oil
dc.creator.none.fl_str_mv Hernández, M. Luisa
Muñoz-Ocaña, Cristina
Posada, Pilar
Sicardo, M. Dolores
Hornero-Méndez, Dámaso
Gómez-Coca, R. B.
Belaj, Angjelina
Moreda, Wenceslao
Martínez-Rivas, José Manuel
author Hernández, M. Luisa
author_facet Hernández, M. Luisa
Muñoz-Ocaña, Cristina
Posada, Pilar
Sicardo, M. Dolores
Hornero-Méndez, Dámaso
Gómez-Coca, R. B.
Belaj, Angjelina
Moreda, Wenceslao
Martínez-Rivas, José Manuel
author_role author
author2 Muñoz-Ocaña, Cristina
Posada, Pilar
Sicardo, M. Dolores
Hornero-Méndez, Dámaso
Gómez-Coca, R. B.
Belaj, Angjelina
Moreda, Wenceslao
Martínez-Rivas, José Manuel
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia, Innovación y Universidades (España)
Agencia Estatal de Investigación (España)
European Commission
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Olea europaea
SQS
Core collection
Gene expression
Olive fruit
Water stress
topic Olea europaea
SQS
Core collection
Gene expression
Olive fruit
Water stress
description 12 Páginas.-- 6 Figuras
publishDate 2023
dc.date.none.fl_str_mv 2023
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/338413
https://api.elsevier.com/content/abstract/scopus_id/85175218986
url http://hdl.handle.net/10261/338413
https://api.elsevier.com/content/abstract/scopus_id/85175218986
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-87871-R
Journal of agricultural and food chemistry
https://doi.org/10.1021/acs.jafc.3c05322
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,81155

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