Datasets including controls for Vfr effect on growth, biofilm, EPS and motility of A. baumannii [Dataset]

Growth (A), biofilm formation (B), EPS production (C) and motility (D) of cavA and vfr related strains demonstrating that the observed phenotypes were not attributed to growth alternations of the strains. Moreover, the empty miniTn7 system (EV) with Tetracycline (Tc) or Tellurate (Tel) resistance ma...

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Bibliographic Details
Authors: Harkova, Lyuboslava G., Dios, Rubén de, Rubio, Alejandro, Pérez-Pulido, Antonio J., McCarthy, Ronan R.
Format: conjunto de datos
Status:Published version
Publication Date:2024
Country:España
Institution:Consejo Superior de Investigaciones Científicas (CSIC)
Repository:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/373203
Online Access:http://hdl.handle.net/10261/373203
Access Level:Open access
Keyword:Throughput screening approach
Modulating quorum sensing
Current treatment options
Baumannii &# 8217
Ultimately governing virulence
Adaptive antibiotic resistance
Opportunistic nosocomial pathogen
High potential target
Acinetobacter baumannii </
Ac ), cava
Baumannii </
Virulence regulation
Increasing resistance
Cava ac
Vivo </
Various aspects
Using drna
Study uncovers
Signalling cascade
Second messengers
Resort antibiotics
Pathogenic success
Mortality rates
Leading position
Inversely regulated
Intrabacterial signalling
High morbidity
Gmp systems
First time
Exopolysaccharide production
Established paradigm
Directly proportional
Cyclic di
Cyclic amp gy
Crucial mediator
Central regulator
Bacterial physiology
Description
Summary:Growth (A), biofilm formation (B), EPS production (C) and motility (D) of cavA and vfr related strains demonstrating that the observed phenotypes were not attributed to growth alternations of the strains. Moreover, the empty miniTn7 system (EV) with Tetracycline (Tc) or Tellurate (Tel) resistance markers, used for the complementations and genes expression in different backgrounds, did not have an effect on any of the tested phenotypes. Growth and biofilm formation were assessed after 24 h at 37°C shaking. EPS production was assessed on Congo agar after 5 days incubation of the strains at 37°C and motility was tested after 48 h on soft agar at 37°C. ns p>0.05, *p<0.05, ** p<0.01, *** p<0.001, **** p<0.0001—One-Way ANOVA with Tukey post-hoc test.

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