CavA and Vfr are regulators of global intracellular c-di-GMP levels in A. baumannii [Dataset]
Cyclic di-GMP levels measured using CensYBL-Ab biosensor in ΔcavA mutant with low cAMP and ΔcavA+cavA complemented strain with high cAMP levels (A) as well as vfr::Tn mutant (B) compared to WT AB5075. Deletion of cavA reduced c-di-GMP levels by 66% (A) while disruption of vfr decreased them by 49% (...
| Autores: | , , , , |
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| Tipo de recurso: | conjunto de datos |
| Estado: | Versión publicada |
| Fecha de publicación: | 2024 |
| País: | España |
| Institución: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/372404 |
| Acceso en línea: | http://hdl.handle.net/10261/372404 |
| Access Level: | acceso abierto |
| Palabra clave: | Throughput screening approach Modulating quorum sensing Current treatment options Baumannii &# 8217 Ultimately governing virulence Adaptive antibiotic resistance Opportunistic nosocomial pathogen High potential target Acinetobacter baumannii </ Ac ), cava Baumannii </ Virulence regulation Increasing resistance Cava ac Vivo </ Various aspects Using drna Study uncovers Signalling cascade Second messengers Resort antibiotics Pathogenic success Mortality rates Leading position Inversely regulated Intrabacterial signalling High morbidity Gmp systems First time Exopolysaccharide production Established paradigm Directly proportional Cyclic di Cyclic amp gy Crucial mediator Central regulator Bacterial physiology |
| Sumario: | Cyclic di-GMP levels measured using CensYBL-Ab biosensor in ΔcavA mutant with low cAMP and ΔcavA+cavA complemented strain with high cAMP levels (A) as well as vfr::Tn mutant (B) compared to WT AB5075. Deletion of cavA reduced c-di-GMP levels by 66% (A) while disruption of vfr decreased them by 49% (B). Cultures were diluted in LB broth supplemented with apramycin (100 μg/ml) and biosensor expression was induced with anhydrotetracycline (50 ng/ml). Harvested cells were resuspended in sterile PBS and YFP and mCherry fluorescence signals were measured. The average normalised fluorescence (YFP/mCherry) ± SD from three independent biological repeats is presented. Data was analysed using One-Way ANOVA with Tukey post-hoc test (A) and Unpaired t-test (B) (* p<0.05, ** p<0.01). Strains harbouring empty miniTn7 were used as controls (S9B Fig). |
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