PpilA and Ppga transcriptional regulation by CavA and Vfr in A. baumannii [Dataset]
Expression of pilA gene (A) and pga operon (B) is regulated by CavA and Vfr. Promoter regions of pilA and pga fused with gfpmut3 fluorescent reporter (PpilA::gfpmut3 and Ppga::gfpmut3 respectively) were used to assess the effect of CavA and Vfr on their expression. Bacterial cells were harvested fro...
| Autores: | , , , , |
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| Tipo de recurso: | conjunto de datos |
| Estado: | Versión publicada |
| Fecha de publicación: | 2024 |
| País: | España |
| Institución: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/373200 |
| Acceso en línea: | http://hdl.handle.net/10261/373200 |
| Access Level: | acceso abierto |
| Palabra clave: | Throughput screening approach Modulating quorum sensing Current treatment options Baumannii &# 8217 Ultimately governing virulence Adaptive antibiotic resistance Opportunistic nosocomial pathogen High potential target Acinetobacter baumannii </ Ac ), cava Baumannii </ Virulence regulation Increasing resistance Cava ac Vivo </ Various aspects Using drna Study uncovers Signalling cascade Second messengers Resort antibiotics Pathogenic success Mortality rates Leading position Inversely regulated Intrabacterial signalling High morbidity Gmp systems First time Exopolysaccharide production Established paradigm Directly proportional Cyclic di Cyclic amp gy Crucial mediator Central regulator Bacterial physiology |
| Sumario: | Expression of pilA gene (A) and pga operon (B) is regulated by CavA and Vfr. Promoter regions of pilA and pga fused with gfpmut3 fluorescent reporter (PpilA::gfpmut3 and Ppga::gfpmut3 respectively) were used to assess the effect of CavA and Vfr on their expression. Bacterial cells were harvested from diluted bacterial cultures grown for 4.5 h, after which were resuspended in sterile PBS. Fluorescence (A.U.) at 470-15/515-20 nm excitation/emission was measured to determine the expression of Gfp and thus the expression of each promoter. Optical density at 600 nm (OD600) was measured to determine growth. Data represents the average of three independent repeats ± SD. **p<0.01, ****p<0.0001 –One-Way ANOVA with Tukey post-hoc test. |
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