Proliferación de células β pancreáticas en ratones diabéticos tratados con Cucurbita ficifolia Bouché
In Mexico, diabetes mellitus (DM) is one of the leading causes of death due to vascular complications that accompany it, reducing the life expectancy of the patients, and it is considered as a disease with excessive cost for prevention and control. It is estimated that the world has more than 346 mi...
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| Tipo de recurso: | tesis de maestría |
| Estado: | Versión publicada |
| Fecha de publicación: | 2011 |
| País: | México |
| Institución: | Universidad Autónoma Metropolitana |
| Repositorio: | Repositorio Institucional de la UAM Iztapalapa |
| Idioma: | español |
| OAI Identifier: | oai:bindani.izt.uam.mx:m900nt67v |
| Acceso en línea: | https://doi.org/10.24275/uami.m900nt67v |
| Access Level: | acceso abierto |
| Palabra clave: | info:eu-repo/classification/LEM/Medicinal plants info:eu-repo/classification/LEM/Pumpkin info:eu-repo/classification/LEM/Cucurbita ficifolia Bouché info:eu-repo/classification/LEM/Pancreatic beta cells info:eu-repo/classification/LEM/Diabetes -- Treatment info:eu-repo/classification/LEM/Células beta pancreáticas info:eu-repo/classification/LEM/Plantas medicinales info:eu-repo/classification/LEM/Diabetes -- Tratamiento info:eu-repo/classification/cti/3 |
| Sumario: | In Mexico, diabetes mellitus (DM) is one of the leading causes of death due to vascular complications that accompany it, reducing the life expectancy of the patients, and it is considered as a disease with excessive cost for prevention and control. It is estimated that the world has more than 346 million people with diabetes and in 2030 will become the seventh leading cause of death. The DM is characterized by a chronic state of hyperglycemia due to defects in the action and/or secretion of insulin, causing alterations in the metabolism of carbohydrates, lipids and proteins. In particular, type 2 diabetes (DT2) occurs mostly in adulthood and is characterized by resistance and decreased in the secretion of insulin, as resulted of progressive dysfunction of pancreatic β cells, which have a low capacity for regeneration. The cyclin-dependent kinase type 4 (CDK4), which is involved in the cell regeneration capacity, as well as in the growth and biosynthesis of insulin, which regulating cell cycle in the G1 phase, represents one of the most important markers of proliferation of the β cell. The high incidence, prevalence and mortality due to diabetes, as well as its vascular complications, have led to propose new pharmacological alternatives of treatment. Cucurbita ficifolia Bouché (C. ficifolia), a plant used empirically in the control of DT2 in Mexico, has shown hypoglycemic activity in clinical and experimental studies. In addition, some preliminary histological observations suggest the possibility of increased cell-β density in the islets of Langerhans of mouse pancreas. However, these observations require support through morphological and immuno-histochemical quantitative studies. In order to establish the basis for proposing to C.ficifolia like an alternative therapy with capacity of regenerate the pancreas, through the augmenting the proliferation of cells-β pancreatic, which may avoid the development of vascular complications, in the present research a morphological study was performed in liver, kidney and pancreas in normal and diabetic mice treated with C. ficifolia, quantifying the number of islets and its cellular density in pancreas. In addition, the effect on CDK4 (a cell proliferation marker) was evaluated, as well as on the serum concentrations of insulin in the same experimental model. The results showed that C. ficifolia reduces blood glucose levels, correcting some characteristic parameters of DT2. In addition, C. ficifolia increased insulin levels, as well as CDK4 in the pancreatic islets without altering the morphology of this organ. C. ficifolia treated of maintain the overall structure of the liver and kidney. C. ficifolia also modified the distribution of liver glycogen, suggesting that in the mechanism of action of the extract may be involved both the synthesis of insulin by CDK4 as the glycogen synthesis pathway. C. ficifolia may be considered a therapeutic alternative in the treatment of diabetes mellitus, with both liver hypoglycemic effect and pancreatic regenerator, which could slow the development of vascular complications and improve quality of life of patients with diabetes. |
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