Caracterización física, química y fisiológica de frutos de Cucurbita ficifolia Bouché (chilacayote) durante su crecimiento y desarrollo, asociada a su capacidad hipoglucemiante
The fruit of Cucurbita ficifolia Bouché is known in Mexico as chilacayote, it has medicinal properties such as anthelmintic, diuretic, taeniafuge, among others, highlighting its hypoglycemic effect, which has generated great scientific interest since its effect is 12.9% better than Opuntia streptaca...
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| Tipo de recurso: | tesis doctoral |
| Estado: | Versión publicada |
| Fecha de publicación: | 2020 |
| País: | México |
| Institución: | Universidad Autónoma Metropolitana |
| Repositorio: | Repositorio Institucional de la UAM Iztapalapa |
| Idioma: | español |
| OAI Identifier: | oai:bindani.izt.uam.mx:sf268532d |
| Acceso en línea: | https://doi.org/10.24275/uami.sf268532d |
| Access Level: | acceso abierto |
| Palabra clave: | info:eu-repo/classification/LEM/Diabetes info:eu-repo/classification/LEM/Metabolic syndrome info:eu-repo/classification/LEM/Chilacayote info:eu-repo/classification/LEM/Cucurbita ficifolia Bouché info:eu-repo/classification/LEM/Fruit info:eu-repo/classification/LEM/Biotecnología info:eu-repo/classification/LEM/Alimentos funcionales info:eu-repo/classification/LEM/Pumpkin info:eu-repo/classification/LEM/Síndrome metabólico info:eu-repo/classification/LEM/Frutas info:eu-repo/classification/LEM/Functional foods info:eu-repo/classification/cti/6 |
| Sumario: | The fruit of Cucurbita ficifolia Bouché is known in Mexico as chilacayote, it has medicinal properties such as anthelmintic, diuretic, taeniafuge, among others, highlighting its hypoglycemic effect, which has generated great scientific interest since its effect is 12.9% better than Opuntia streptacantha Lem., a species used to control glucose levels and 14.6% better than tolbutamide, a drug used to treat type 2 diabetes mellitus. However, it is unknown if this effect varies according to the stage of development of the fruit and which compounds are present in the extract of C. ficifolia that confer this property. Therefore, the objective of this research was to determine the hypoglycemic effect at different stages of development of these fruits and to characterize the chemical compounds that could be involved. The methodology to achieve this goal was the following: a plot was established where the seedlings obtained of C. ficifolia seeds in postharvest Biotechnology laboratory were transplanted, later the flowers were marked at the time of anthesis and after the full flowering (5 days after transplanting) samples of five fruits were harvested (three replicate) at nine different stages. Ethylene production and respiration rate were determined using gas chromatography, using a FID and a TCD respectively, maturity indices such as weight, longitudinal and equatorial perimeter, volume, firmness, dry matter, acidity, pH and SST were determined. The chemical characterization of the aqueous extracts was performed by HPLC, the content of DQI was quantified using UFLC and the hypoglycemic effect was determined in an acute study in male CD-1 mice diabetized with STZ and normoglycemic. The respiration rate and ethylene production results showed a typical pattern for non–climacteric fruits, the growth pattern found was a simple sigmoid curve and the quality parameters did not show significant changes. Compounds such as catechin, gallic acid, epicatechin, chlorogenic acid, myricetin and syringic acid were found in the aqueous extracts, reaching the highest concentration at 15 days of fruit development, the DQI was found only in the fruits of 45 days of development. All extracts showed a hypoglycemic effect similar to glibenclamide, however, the extract of 15 days had an 11% greater effect than this medicine in STZ diabetic mice. Therefore, C. ficifolia extract at this stage of development may be a good alternative for the treatment of DM2. |
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