Efecto de un extracto hipoglucemiante de Cucurbita ficifolia Bouché sobre los niveles de citocinas proinflamatorias en ratones obesos
The overweight and the obesity are defined as abnormal or excessive fat accumulation that may impair the health. According to the World Health Organization, in 2005 approximately 1.6 billion of adults had overweight, whereas 400 million of adults were obese. Overweight and obesity are considered ris...
| Author: | |
|---|---|
| Format: | master thesis |
| Status: | Published version |
| Publication Date: | 2009 |
| Country: | México |
| Institution: | Universidad Autónoma Metropolitana |
| Repository: | Repositorio Institucional de la UAM Iztapalapa |
| Language: | Spanish |
| OAI Identifier: | oai:bindani.izt.uam.mx:6t053g149 |
| Online Access: | https://doi.org/10.24275/uami.6t053g149 |
| Access Level: | Open access |
| Keyword: | info:eu-repo/classification/LEM/Obesity info:eu-repo/classification/LEM/Obesidad info:eu-repo/classification/LEM/Cucurbits info:eu-repo/classification/LEM/Diabetes -- Treatment info:eu-repo/classification/LEM/Curcubitáceas info:eu-repo/classification/LEM/Diabetes -- Tratamiento info:eu-repo/classification/cti/3 |
| Summary: | The overweight and the obesity are defined as abnormal or excessive fat accumulation that may impair the health. According to the World Health Organization, in 2005 approximately 1.6 billion of adults had overweight, whereas 400 million of adults were obese. Overweight and obesity are considered risk factors for developing type 2 diabetes mellitus. The obesity and diabetes are associated with a low grade-chronic inflammatory response, due to an abnormal production of cytokines. White adipose tissue plays an important role in this inflammatory process. In obesity, the excess of adipose tissue causes an inflammatory condition by abnormal production of cytokines, such as tumor necrosis factor type alpha (TNF-α), interleukin 6 (IL-6), resistin and adiponectin, which have been associated with the generation of insulin resistance. The importance of inflammation in the generation of these pathologies has propitiated the search of new therapies for treatment of this illness. In Mexico, Cucurbita ficifolia is a plant used empirically in type 2 diabetes mellitus control. The aqueous extract from C. ficifolia fruit decreased serum levels of TNF-α and increased the levels of interferon gamma (IFN-γ) and interleukin 10 (IL-10) in diabetic mice. However, the effects of C. ficifolia on the inflammation in obesity have not yet been studied. Because the importance of white adipose tissue in the regulation of cytokine production, in the present research it was proposed to evaluate the effect of daily administration of a hypoglycemic extract from C. ficifolia fruit on the serum levels and the gene expression of inflammatory cytokines in obese mice. An obesity model induced by neonatal administration of monosodium glutamate was used, which presents a characteristic inflammatory state and insulin resistance. Obese mice with 4 months of age received C. ficifolia extract (200mg/kg) for 30 days by gastric gavage. At the end of the treatment serum levels and mRNA expression in adipose tissue for TNF-α, IL-6, resistin, IL-10 and adiponectin were quantified. The results indicated that administration of C. ficifolia significantly decreased serum levels of TNFR2 and IL-6, as well as the mRNA expression of the proinflammatory cytokines resistin, TNF-α and IL-6 (P <0.05). The administration of C. ficifolia did not increase the levels of the anti-inflammatory cytokines IL-10 and adiponectin. Although a significant reduction (P <0.05) of adiponectin mRNA expression was observed. Also C. ficifolia reduced insulin resistance in obese mice (P <0.05). In conclusion, these results suggest that the hypoglycemic extract of C. ficifolia has anti-inflammatory effect in obese mice, resulting in amelioration of the insulin resistance. |
|---|