Efecto de la glicina y Cucurbita ficifolia Bouché sobre la glicación de proteínas
Diabetes mellitus (DM) is a group of metabolic diseases characterized by chronic hyperglycemia, which is due to decreased secretion and/or activity of insulin, the hormone released by pancreatic β cells. During periods of hyperglycaemia, the hexoses react with proteins to form advanced glycation pro...
| Autor: | |
|---|---|
| Tipo de recurso: | tesis de maestría |
| Estado: | Versión publicada |
| Fecha de publicación: | 2009 |
| País: | México |
| Institución: | Universidad Autónoma Metropolitana |
| Repositorio: | Repositorio Institucional de la UAM Iztapalapa |
| Idioma: | español |
| OAI Identifier: | oai:bindani.izt.uam.mx:8w32r5837 |
| Acceso en línea: | https://doi.org/10.24275/uami.8w32r5837 |
| Access Level: | acceso abierto |
| Palabra clave: | info:eu-repo/classification/LEM/Curcubitáceas info:eu-repo/classification/LEM/Glicina -- Efectos fisiológicos info:eu-repo/classification/LEM/Diabetes -- Treatment info:eu-repo/classification/LEM/Glycine -- Physiological effect info:eu-repo/classification/LEM/Diabetes -- Tratamiento info:eu-repo/classification/LEM/Cucurbits info:eu-repo/classification/cti/3 |
| Sumario: | Diabetes mellitus (DM) is a group of metabolic diseases characterized by chronic hyperglycemia, which is due to decreased secretion and/or activity of insulin, the hormone released by pancreatic β cells. During periods of hyperglycaemia, the hexoses react with proteins to form advanced glycation products (AGES), favoring the development of pathologic complications that jeopardize the health of individuals and accelerate the aging process. As a direct consequence of glycation, the biological activity of various physiologically important proteins is affected. Due to the negative impact of DM on quality of life of the patients, it is necessary to have alternatives that directly avoid the glycation of proteins. These alternatives would be helpful to prevent the development of such complications. Recent studies in laboratory animals and diabetic patients indicate that the intake of glycine reduces the levels of glycated hemoglobin (A1c). Furthermore, some people in Mexico also use medicinal plants for DM control. One of them is Cucurbita ficifolia, popularly known as “chilacayote” and whose fruits are edible. It has been experimentally and clinically demonstrated that the fruit of this plant has hypoglycaemic effect through stimulating the secretion of insulin, consequently reducing levels of glycated hemoglobin. Although glycine and C. ficifolia represent alternatives that potentially could reduce the glycation of other proteins, studies conducted about this have not yet been performed. The objective of this research was to determine the effect of the daily administration of glycine and an aqueous extract of C. ficifolia on the glycation of proteins (hemoglobin, albumin and cholynesterases) in normal and diabetic mice. Male mice of strain CD‐1 were used. Three groups were formed of healthy animals and other of diabetic animals (n = 5), which received the following treatments for 71 and 21 days, respectively: Group 1: control, Group 2 glycine (0.1 g / kg), Group 3 C. ficifolia (0.65 mg/kg). Glucose was measured with and without food. In addition, triglycerides, cholesterol, and the activity of glutamic oxalacetic and pyruvic transaminases activities (GOT and GPT, respectively) were determined. Curves of oral glucose tolerance in healthy mice were also performed. The level of glycation in hemoglobin, albumin and cholinesterase were finally registered. Results in healthy mice showed that both treatments reduced blood glucose in a glucose tolerance curve. In diabetic animals treated with glycine it was shown that there was a 1.3% of reduction in levels of glycated albumin. In the case of the extract, its administration resulted in a 1.1% decrease in glycated hemoglobin levels. The glycation index on cholinesterases in the treated diabetic mice indicated an elevation in the glycation of acetycholinesterase but no in butyrylcholinesterase. Finally, mice showed significant changes in the activity of cholinesterases with both treatments. Therefore, both treatments (C. ficifolia and glycine) impaired the glycation of different proteins. |
|---|