Genotyping, identification and multifunctional features of yeasts associated to Bosana naturally black table olive fermentations

31 Páginas; 4 Figuras

Detalles Bibliográficos
Autores: Porru, Cinzia, Rodríguez-Gómez, Francisco J., Benítez-Cabello, Antonio, Jiménez Díaz, Rufino, Zara, Giacomo, Budroni, Marilena, Mannazzu, Ilaria, Arroyo López, Francisco Noé
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2017
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/157877
Acceso en línea:http://hdl.handle.net/10261/157877
Access Level:acceso abierto
Palabra clave:Biofilms
Olive fermentations
Multifunctional starter
Yeast community
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spelling Genotyping, identification and multifunctional features of yeasts associated to Bosana naturally black table olive fermentationsPorru, CinziaRodríguez-Gómez, Francisco J.Benítez-Cabello, AntonioJiménez Díaz, RufinoZara, GiacomoBudroni, MarilenaMannazzu, IlariaArroyo López, Francisco NoéBiofilmsOlive fermentationsMultifunctional starterYeast community31 Páginas; 4 FigurasDirectly brined black table olives of Bosana variety are a traditional food product of Sardinia island (Italy), spontaneously fermented by yeasts among other microorganisms. However, as far as we know, the identification, biotechnological and probiotic potential of this yeast community has not been investigated yet. In this work, a total of 72 yeast isolates previously obtained from Bosana olive brines were first genotyped by Random Amplified Polymorphic DNA (RAPD-PCR) analysis with primer M13, and then identified by sequencing of D1/D2 domains of rDNA 26S gene. The dominant species were Wickerhamomyces anomalus and Nakazawaea molendini-olei, albeit Candida diddensiae, Candida boidinii, Zygotorulaspora mrakii, and Saccharomyces cerevisiae were also present in lower proportions. For the different biotypes of yeasts obtained, the multivariate analysis of their technological (esterase, lipase and β-glucosidase activities, growth in presence of oleuropein, resistance and susceptibility to NaCl) and probiotic (removal of cholesterol, gastric and pancreatic digestions, biofilms assays alone and in co-culture with Lactobacillus pentosus) features, showed that W. anomalus Wa1 exhibited the best technological characteristics, while S. cerevisiae Sc24 and C. boidinii Cb60 showed promising probiotic features. Therefore, they may have potential application as multifunctional starters, alone or in combination with lactic acid bacteria, during olive processing, albeit further studies are necessary to validate these results.The research leading to these results has received funding from the Spanish Government (Project OliFilm AGL-2013-48300-R: www.olifilm.science.com.es), and Junta de Andalucía (through financial support to projects P11-AGR-7051, P11-AGR-7755, and groups AGR-125 and BIO-160). CP gratefully acknowledges Sardinia regional Government for the financial support of her PhD scholarship (POR Sardegna FSE OP European Social Fund2007/2013 – Axis IV Human Resources, Objective 1.3, Line of activity 1.3.1) and the Foundation of Sardinia (Prat. 2013.1364) for financial support. FNAL wish to express thanks to the Spanish Government for his postdoctoral research contract (Ramón y Cajal). Authors express their gratitude to Dr. Antonio Garrido Fernández for his constructive comments and suggestions with drafting of manuscript. CP gratetfully acknowledges Dr Ivo Pinna for kindly provided support of her thesis manuscript.Peer reviewedElsevierMinisterio de Economía y Competitividad (España)Junta de AndalucíaConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201720172018info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/157877reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#MINECI/ICTI2013-2016/AGL-2013-48300-Rhttp://dx.doi.org/10.1016/j.fm.2017.07.010Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1578772026-05-22T06:33:51Z
dc.title.none.fl_str_mv Genotyping, identification and multifunctional features of yeasts associated to Bosana naturally black table olive fermentations
title Genotyping, identification and multifunctional features of yeasts associated to Bosana naturally black table olive fermentations
spellingShingle Genotyping, identification and multifunctional features of yeasts associated to Bosana naturally black table olive fermentations
Porru, Cinzia
Biofilms
Olive fermentations
Multifunctional starter
Yeast community
title_short Genotyping, identification and multifunctional features of yeasts associated to Bosana naturally black table olive fermentations
title_full Genotyping, identification and multifunctional features of yeasts associated to Bosana naturally black table olive fermentations
title_fullStr Genotyping, identification and multifunctional features of yeasts associated to Bosana naturally black table olive fermentations
title_full_unstemmed Genotyping, identification and multifunctional features of yeasts associated to Bosana naturally black table olive fermentations
title_sort Genotyping, identification and multifunctional features of yeasts associated to Bosana naturally black table olive fermentations
dc.creator.none.fl_str_mv Porru, Cinzia
Rodríguez-Gómez, Francisco J.
Benítez-Cabello, Antonio
Jiménez Díaz, Rufino
Zara, Giacomo
Budroni, Marilena
Mannazzu, Ilaria
Arroyo López, Francisco Noé
author Porru, Cinzia
author_facet Porru, Cinzia
Rodríguez-Gómez, Francisco J.
Benítez-Cabello, Antonio
Jiménez Díaz, Rufino
Zara, Giacomo
Budroni, Marilena
Mannazzu, Ilaria
Arroyo López, Francisco Noé
author_role author
author2 Rodríguez-Gómez, Francisco J.
Benítez-Cabello, Antonio
Jiménez Díaz, Rufino
Zara, Giacomo
Budroni, Marilena
Mannazzu, Ilaria
Arroyo López, Francisco Noé
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
Junta de Andalucía
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Biofilms
Olive fermentations
Multifunctional starter
Yeast community
topic Biofilms
Olive fermentations
Multifunctional starter
Yeast community
description 31 Páginas; 4 Figuras
publishDate 2017
dc.date.none.fl_str_mv 2017
2017
2018
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/157877
url http://hdl.handle.net/10261/157877
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
MINECI/ICTI2013-2016/AGL-2013-48300-R
http://dx.doi.org/10.1016/j.fm.2017.07.010
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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