Survey of the analysis of continuous conformational variability of biological macromolecules by electron microscopy

Single-particle analysis by electron microscopy is a well established technique for analyzing the three-dimensional structures of biological macromolecules. Besides its ability to produce high-resolution structures, it also provides insights into the dynamic behavior of the structures by elucidating...

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Autores: Sorzano, Carlos Óscar S., Jiménez, A., Vilas, J. L., Maluenda, D., Martínez, Marta, Ramírez-Aportela, Erney, Majtner, T., Segura, J., Sánchez-García, Rubén, Caño, Laura del, Conesa Mingo, Pablo, Melero, Roberto, Jonic, Slavica, Vargas, Javier, Cazals, F., Freyberg, Z., Krieger, James, Bahar, Ivet, Marabini, Roberto, Carazo, José M.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/193093
Acceso en línea:http://hdl.handle.net/10261/193093
Access Level:acceso abierto
Palabra clave:Electron microscopy
Image processing
Continuous heterogeneity
Single-particle analysis
Normal-mode analysis
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spelling Survey of the analysis of continuous conformational variability of biological macromolecules by electron microscopySorzano, Carlos Óscar S.Jiménez, A.Vilas, J. L.Maluenda, D.Martínez, MartaRamírez-Aportela, ErneyMajtner, T.Segura, J.Sánchez-García, RubénCaño, Laura delConesa Mingo, PabloMelero, RobertoJonic, SlavicaVargas, JavierCazals, F.Freyberg, Z.Krieger, JamesBahar, IvetMarabini, RobertoCarazo, José M.Electron microscopyImage processingContinuous heterogeneitySingle-particle analysisNormal-mode analysisSingle-particle analysis by electron microscopy is a well established technique for analyzing the three-dimensional structures of biological macromolecules. Besides its ability to produce high-resolution structures, it also provides insights into the dynamic behavior of the structures by elucidating their conformational variability. Here, the different image-processing methods currently available to study continuous conformational changes are reviewed.Spanish Ministry of Economy and Competitiveness through grants BIO2013-44647-R and BIO2016-76400-R (AEI/FEDER, UE), Comunidad Autonoma de Madrid through grant S2017/BMD-3817, Instituto de Salud Carlos III through grants PT13/0001/0009 and PT17/0009/0010, the European Union (EU) and Horizon 2020 through West-Life (EINFRA-2015-1, Proposal 675858), CORBEL (INFRADEV-1-2014-1, Proposal 654248), ELIXIR–EXCELERATE (INFRADEV-3-2015, Proposal 676559), iNEXT (INFRAIA-1-2014-2015, Proposal 653706), EOSCpilot (INFRADEV-04-2016, Proposal 739563) and the National Institutes of Health (P41GM 103712) (IB).Peer reviewedJohn Wiley & SonsMinisterio de Economía y Competitividad (España)Comunidad de MadridInstituto de Salud Carlos IIINational Institutes of Health (US)European CommissionCarazo, J. M [0000-0003-0788-8447]Sorzano, Carlos Óscar S. [0000-0002-9473-283X]Marabini, Roberto [0000-0001-7876-1684]Melero, Roberto [0000-0001-9467-9381]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201920192019info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/193093reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1107/S2053230X18015108Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1930932026-05-22T06:33:51Z
dc.title.none.fl_str_mv Survey of the analysis of continuous conformational variability of biological macromolecules by electron microscopy
title Survey of the analysis of continuous conformational variability of biological macromolecules by electron microscopy
spellingShingle Survey of the analysis of continuous conformational variability of biological macromolecules by electron microscopy
Sorzano, Carlos Óscar S.
Electron microscopy
Image processing
Continuous heterogeneity
Single-particle analysis
Normal-mode analysis
title_short Survey of the analysis of continuous conformational variability of biological macromolecules by electron microscopy
title_full Survey of the analysis of continuous conformational variability of biological macromolecules by electron microscopy
title_fullStr Survey of the analysis of continuous conformational variability of biological macromolecules by electron microscopy
title_full_unstemmed Survey of the analysis of continuous conformational variability of biological macromolecules by electron microscopy
title_sort Survey of the analysis of continuous conformational variability of biological macromolecules by electron microscopy
dc.creator.none.fl_str_mv Sorzano, Carlos Óscar S.
Jiménez, A.
Vilas, J. L.
Maluenda, D.
Martínez, Marta
Ramírez-Aportela, Erney
Majtner, T.
Segura, J.
Sánchez-García, Rubén
Caño, Laura del
Conesa Mingo, Pablo
Melero, Roberto
Jonic, Slavica
Vargas, Javier
Cazals, F.
Freyberg, Z.
Krieger, James
Bahar, Ivet
Marabini, Roberto
Carazo, José M.
author Sorzano, Carlos Óscar S.
author_facet Sorzano, Carlos Óscar S.
Jiménez, A.
Vilas, J. L.
Maluenda, D.
Martínez, Marta
Ramírez-Aportela, Erney
Majtner, T.
Segura, J.
Sánchez-García, Rubén
Caño, Laura del
Conesa Mingo, Pablo
Melero, Roberto
Jonic, Slavica
Vargas, Javier
Cazals, F.
Freyberg, Z.
Krieger, James
Bahar, Ivet
Marabini, Roberto
Carazo, José M.
author_role author
author2 Jiménez, A.
Vilas, J. L.
Maluenda, D.
Martínez, Marta
Ramírez-Aportela, Erney
Majtner, T.
Segura, J.
Sánchez-García, Rubén
Caño, Laura del
Conesa Mingo, Pablo
Melero, Roberto
Jonic, Slavica
Vargas, Javier
Cazals, F.
Freyberg, Z.
Krieger, James
Bahar, Ivet
Marabini, Roberto
Carazo, José M.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
Comunidad de Madrid
Instituto de Salud Carlos III
National Institutes of Health (US)
European Commission
Carazo, J. M [0000-0003-0788-8447]
Sorzano, Carlos Óscar S. [0000-0002-9473-283X]
Marabini, Roberto [0000-0001-7876-1684]
Melero, Roberto [0000-0001-9467-9381]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Electron microscopy
Image processing
Continuous heterogeneity
Single-particle analysis
Normal-mode analysis
topic Electron microscopy
Image processing
Continuous heterogeneity
Single-particle analysis
Normal-mode analysis
description Single-particle analysis by electron microscopy is a well established technique for analyzing the three-dimensional structures of biological macromolecules. Besides its ability to produce high-resolution structures, it also provides insights into the dynamic behavior of the structures by elucidating their conformational variability. Here, the different image-processing methods currently available to study continuous conformational changes are reviewed.
publishDate 2019
dc.date.none.fl_str_mv 2019
2019
2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/193093
url http://hdl.handle.net/10261/193093
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1107/S2053230X18015108
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv John Wiley & Sons
publisher.none.fl_str_mv John Wiley & Sons
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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