Algorithmic robustness to preferred orientations in single particle analysis by CryoEM

The presence of preferred orientations in single particle analysis (SPA) by cryo-Electron Microscopy (cryoEM) is currently one of the hurdles preventing many structural analyses from yielding high-resolution structures. Although the existence of preferred orientations is mostly related to the grid p...

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Detalles Bibliográficos
Autores: Sorzano, C. O.S., Semchonok, D., Lin, S. C., Lo, Y. C., Vilas, J. L., Jiménez-Moreno, A., Gragera, M., Vacca, S., Maluenda, D., Martínez, M., Ramírez-Aportela, E., Melero, R., Cuervo, A., Conesa, J. J., Conesa, P., Losana, P., Caño, L. del, de la Morena, J. Jiménez, Fonseca, Y. C., Sánchez-García, R., Strelak, D., Fernández-Giménez, E., de Isidro, F., Herreros, D., Kastritis, P. L., Bruce, B. D., Carazo, J. M., Marabini Ruiz, Roberto
Tipo de recurso: artículo
Fecha de publicación:2021
País:España
Institución:Universidad Autónoma de Madrid
Repositorio:Biblos-e Archivo. Repositorio Institucional de la UAM
Idioma:inglés
OAI Identifier:oai:repositorio.uam.es:10486/701359
Acceso en línea:http://hdl.handle.net/10486/701359
https://dx.doi.org/10.1016/j.jsb.2020.107695
Access Level:acceso abierto
Palabra clave:Electron microscopy
Preferred orientations
Single particle analysis
Informática
Descripción
Sumario:The presence of preferred orientations in single particle analysis (SPA) by cryo-Electron Microscopy (cryoEM) is currently one of the hurdles preventing many structural analyses from yielding high-resolution structures. Although the existence of preferred orientations is mostly related to the grid preparation, in this technical note, we show that some image processing algorithms used for angular assignment and three-dimensional (3D) reconstruction are more robust than others to these detrimental conditions. We exemplify this argument with three different data sets in which the presence of preferred orientations hindered achieving a 3D reconstruction without artifacts or, even worse, a 3D reconstruction could never be achieved

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