Survey of the analysis of continuous conformational variability of biological macromolecules by electron microscopy

Single-particle analysis by electron microscopy is a well established technique for analyzing the three-dimensional structures of biological macromolecules. Besides its ability to produce high-resolution structures, it also provides insights into the dynamic behavior of the structures by elucidating...

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Detalles Bibliográficos
Autores: Sorzano, Carlos Óscar S., Jiménez, A., Vilas, J. L., Maluenda, D., Martínez, Marta, Ramírez-Aportela, Erney, Majtner, T., Segura, J., Sánchez-García, Rubén, Caño, Laura del, Conesa Mingo, Pablo, Melero, Roberto, Jonic, Slavica, Vargas, Javier, Cazals, F., Freyberg, Z., Krieger, James, Bahar, Ivet, Marabini, Roberto, Carazo, José M.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/193093
Acceso en línea:http://hdl.handle.net/10261/193093
Access Level:acceso abierto
Palabra clave:Electron microscopy
Image processing
Continuous heterogeneity
Single-particle analysis
Normal-mode analysis
Descripción
Sumario:Single-particle analysis by electron microscopy is a well established technique for analyzing the three-dimensional structures of biological macromolecules. Besides its ability to produce high-resolution structures, it also provides insights into the dynamic behavior of the structures by elucidating their conformational variability. Here, the different image-processing methods currently available to study continuous conformational changes are reviewed.

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