Fluorescence in situ hybridization (FISH) of TP53 for the detection of chromosome 17 abnormalities in myelodysplastic syndromes

Sánchez Castro, Judit; Marco Betés, Victor; Gómez Arbonés, Javier; García Cerecedo, Tomás; López Ortega, Ricard; Talavera, Elisabeth; Fernández-Ruiz, Sara; Ademà, Vera; Marugan, Isabel; Luño, Elisa; Sanzo, Carmen; Vallespí, Teresa; Arenillas, Leonor; Marco Buades, Josefa; Batlle, Ana; Buño, Ismael; Martín Ramos, María Luisa; Blázquez Rios, Beatriz; Collado Nieto, Rosa; Vargas, María Teresa; González Martínez, Teresa; Sanz, Guillermo; Solé, Francesc

Fluorescence in situ hybridization (FISH) of TP53 for the detection of chromosome 17 abnormalities in myelodysplastic syndromes

Conventional G-banding cytogenetics (CC) detects chromosome 17 (chr17) abnormalities in 2% of patients with de novo myelodysplastic syndromes (MDS). We used CC and fluorescence in situ hybridization (FISH) (LSI p53/17p13.1) to assess deletion of 17p in 531 patients with de novo MDS from the Spanish...

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Detalhes bibliográficos
Autores:	Sánchez Castro, Judit, Marco Betés, Victor, Gómez Arbonés, Javier, García Cerecedo, Tomás, López Ortega, Ricard, Talavera, Elisabeth, Fernández-Ruiz, Sara, Ademà, Vera, Marugan, Isabel, Luño, Elisa, Sanzo, Carmen, Vallespí, Teresa, Arenillas, Leonor, Marco Buades, Josefa, Batlle, Ana, Buño, Ismael, Martín Ramos, María Luisa, Blázquez Rios, Beatriz, Collado Nieto, Rosa, Vargas, María Teresa, González Martínez, Teresa, Sanz, Guillermo, Solé, Francesc
Formato:	artículo
Estado:	Versión aceptada para publicación
Fecha de publicación:	2015
País:	España
Recursos:	Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:	Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:	oai:recercat.cat:10459.1/48614
Acesso em linha:	https://doi.org/10.3109/10428194.2015.1028053 http://hdl.handle.net/10459.1/48614
Access Level:	acceso abierto
Palavra-chave:	Myelodysplastic syndromes Cytogenetics FISH Chromosome 17

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spelling	Fluorescence in situ hybridization (FISH) of TP53 for the detection of chromosome 17 abnormalities in myelodysplastic syndromesSánchez Castro, JuditMarco Betés, VictorGómez Arbonés, JavierGarcía Cerecedo, TomásLópez Ortega, RicardTalavera, ElisabethFernández-Ruiz, SaraAdemà, VeraMarugan, IsabelLuño, ElisaSanzo, CarmenVallespí, TeresaArenillas, LeonorMarco Buades, JosefaBatlle, AnaBuño, IsmaelMartín Ramos, María LuisaBlázquez Rios, BeatrizCollado Nieto, RosaVargas, María TeresaGonzález Martínez, TeresaSanz, GuillermoSolé, FrancescMyelodysplastic syndromesCytogeneticsFISHChromosome 17Conventional G-banding cytogenetics (CC) detects chromosome 17 (chr17) abnormalities in 2% of patients with de novo myelodysplastic syndromes (MDS). We used CC and fluorescence in situ hybridization (FISH) (LSI p53/17p13.1) to assess deletion of 17p in 531 patients with de novo MDS from the Spanish Group of Hematological Cytogenetics. FISH detected<br>17 or 17p abnormalities in 13 cases (2.6%) in whom no 17p abnormalities were revealed by CC: 9% of patients with a normal karyotype, 0% in non-informative cytogenetics, 50% of patients with a chr17 abnormality without loss of 17p and in 4.7% of cases with an abnormal karyotype not involving chr17. Our results suggest that applying FISH of 17p13 to identify the number of copies of the TP53 gene could be beneficial in patients with a complex karyotype. We recommend using FISH of 17p13 in young patients with a normal karyotype or non-informative cytogenetics, and always in isolated del(17p)Informa Healthcare2015201620152015info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionapplication/pdfhttps://doi.org/10.3109/10428194.2015.1028053http://hdl.handle.net/10459.1/48614http://hdl.handle.net/10459.1/48614reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésVersió postprint del document publicat a: https://doi.org/10.3109/10428194.2015.1028053Leukemia & Lymphoma, 2015(c) Informa Healthcare, 2015info:eu-repo/semantics/openAccessoai:recercat.cat:10459.1/486142026-05-29T05:05:01Z
dc.title.none.fl_str_mv	Fluorescence in situ hybridization (FISH) of TP53 for the detection of chromosome 17 abnormalities in myelodysplastic syndromes
title	Fluorescence in situ hybridization (FISH) of TP53 for the detection of chromosome 17 abnormalities in myelodysplastic syndromes
spellingShingle	Fluorescence in situ hybridization (FISH) of TP53 for the detection of chromosome 17 abnormalities in myelodysplastic syndromes Sánchez Castro, Judit Myelodysplastic syndromes Cytogenetics FISH Chromosome 17
title_short	Fluorescence in situ hybridization (FISH) of TP53 for the detection of chromosome 17 abnormalities in myelodysplastic syndromes
title_full	Fluorescence in situ hybridization (FISH) of TP53 for the detection of chromosome 17 abnormalities in myelodysplastic syndromes
title_fullStr	Fluorescence in situ hybridization (FISH) of TP53 for the detection of chromosome 17 abnormalities in myelodysplastic syndromes
title_full_unstemmed	Fluorescence in situ hybridization (FISH) of TP53 for the detection of chromosome 17 abnormalities in myelodysplastic syndromes
title_sort	Fluorescence in situ hybridization (FISH) of TP53 for the detection of chromosome 17 abnormalities in myelodysplastic syndromes
dc.creator.none.fl_str_mv	Sánchez Castro, Judit Marco Betés, Victor Gómez Arbonés, Javier García Cerecedo, Tomás López Ortega, Ricard Talavera, Elisabeth Fernández-Ruiz, Sara Ademà, Vera Marugan, Isabel Luño, Elisa Sanzo, Carmen Vallespí, Teresa Arenillas, Leonor Marco Buades, Josefa Batlle, Ana Buño, Ismael Martín Ramos, María Luisa Blázquez Rios, Beatriz Collado Nieto, Rosa Vargas, María Teresa González Martínez, Teresa Sanz, Guillermo Solé, Francesc
author	Sánchez Castro, Judit
author_facet	Sánchez Castro, Judit Marco Betés, Victor Gómez Arbonés, Javier García Cerecedo, Tomás López Ortega, Ricard Talavera, Elisabeth Fernández-Ruiz, Sara Ademà, Vera Marugan, Isabel Luño, Elisa Sanzo, Carmen Vallespí, Teresa Arenillas, Leonor Marco Buades, Josefa Batlle, Ana Buño, Ismael Martín Ramos, María Luisa Blázquez Rios, Beatriz Collado Nieto, Rosa Vargas, María Teresa González Martínez, Teresa Sanz, Guillermo Solé, Francesc
author_role	author
author2	Marco Betés, Victor Gómez Arbonés, Javier García Cerecedo, Tomás López Ortega, Ricard Talavera, Elisabeth Fernández-Ruiz, Sara Ademà, Vera Marugan, Isabel Luño, Elisa Sanzo, Carmen Vallespí, Teresa Arenillas, Leonor Marco Buades, Josefa Batlle, Ana Buño, Ismael Martín Ramos, María Luisa Blázquez Rios, Beatriz Collado Nieto, Rosa Vargas, María Teresa González Martínez, Teresa Sanz, Guillermo Solé, Francesc
author2_role	author author author author author author author author author author author author author author author author author author author author author author
dc.subject.none.fl_str_mv	Myelodysplastic syndromes Cytogenetics FISH Chromosome 17
topic	Myelodysplastic syndromes Cytogenetics FISH Chromosome 17
description	Conventional G-banding cytogenetics (CC) detects chromosome 17 (chr17) abnormalities in 2% of patients with de novo myelodysplastic syndromes (MDS). We used CC and fluorescence in situ hybridization (FISH) (LSI p53/17p13.1) to assess deletion of 17p in 531 patients with de novo MDS from the Spanish Group of Hematological Cytogenetics. FISH detected<br>17 or 17p abnormalities in 13 cases (2.6%) in whom no 17p abnormalities were revealed by CC: 9% of patients with a normal karyotype, 0% in non-informative cytogenetics, 50% of patients with a chr17 abnormality without loss of 17p and in 4.7% of cases with an abnormal karyotype not involving chr17. Our results suggest that applying FISH of 17p13 to identify the number of copies of the TP53 gene could be beneficial in patients with a complex karyotype. We recommend using FISH of 17p13 in young patients with a normal karyotype or non-informative cytogenetics, and always in isolated del(17p)
publishDate	2015
dc.date.none.fl_str_mv	2015 2015 2015 2016
dc.type.none.fl_str_mv	info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion
format	article
status_str	acceptedVersion
dc.identifier.none.fl_str_mv	https://doi.org/10.3109/10428194.2015.1028053 http://hdl.handle.net/10459.1/48614 http://hdl.handle.net/10459.1/48614
url	https://doi.org/10.3109/10428194.2015.1028053 http://hdl.handle.net/10459.1/48614
dc.language.none.fl_str_mv	Inglés
language_invalid_str_mv	Inglés
dc.relation.none.fl_str_mv	Versió postprint del document publicat a: https://doi.org/10.3109/10428194.2015.1028053 Leukemia & Lymphoma, 2015
dc.rights.none.fl_str_mv	(c) Informa Healthcare, 2015 info:eu-repo/semantics/openAccess
rights_invalid_str_mv	(c) Informa Healthcare, 2015
eu_rights_str_mv	openAccess
dc.format.none.fl_str_mv	application/pdf
dc.publisher.none.fl_str_mv	Informa Healthcare
publisher.none.fl_str_mv	Informa Healthcare
dc.source.none.fl_str_mv	reponame:Recercat. Dipósit de la Recerca de Catalunya instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str	Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str	Recercat. Dipósit de la Recerca de Catalunya
collection	Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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Fluorescence in situ hybridization (FISH) of TP53 for the detection of chromosome 17 abnormalities in myelodysplastic syndromes

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