Fluorescence in situ hybridization (FISH) of TP53 for the detection of chromosome 17 abnormalities in myelodysplastic syndromes

Conventional G-banding cytogenetics (CC) detects chromosome 17 (chr17) abnormalities in 2% of patients with de novo myelodysplastic syndromes (MDS). We used CC and fluorescence in situ hybridization (FISH) (LSI p53/17p13.1) to assess deletion of 17p in 531 patients with de novo MDS from the Spanish...

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Detalles Bibliográficos
Autores: Sánchez Castro, Judit, Marco Betés, Victor, Gómez Arbonés, Javier, García Cerecedo, Tomás, López Ortega, Ricard, Talavera, Elisabeth, Fernández-Ruiz, Sara, Ademà, Vera, Marugan, Isabel, Luño, Elisa, Sanzo, Carmen, Vallespí, Teresa, Arenillas, Leonor, Marco Buades, Josefa, Batlle, Ana, Buño, Ismael, Martín Ramos, María Luisa, Blázquez Rios, Beatriz, Collado Nieto, Rosa, Vargas, María Teresa, González Martínez, Teresa, Sanz, Guillermo, Solé, Francesc
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2015
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10459.1/48614
Acceso en línea:https://doi.org/10.3109/10428194.2015.1028053
http://hdl.handle.net/10459.1/48614
Access Level:acceso abierto
Palabra clave:Myelodysplastic syndromes
Cytogenetics
FISH
Chromosome 17
Descripción
Sumario:Conventional G-banding cytogenetics (CC) detects chromosome 17 (chr17) abnormalities in 2% of patients with de novo myelodysplastic syndromes (MDS). We used CC and fluorescence in situ hybridization (FISH) (LSI p53/17p13.1) to assess deletion of 17p in 531 patients with de novo MDS from the Spanish Group of Hematological Cytogenetics. FISH detected<br>17 or 17p abnormalities in 13 cases (2.6%) in whom no 17p abnormalities were revealed by CC: 9% of patients with a normal karyotype, 0% in non-informative cytogenetics, 50% of patients with a chr17 abnormality without loss of 17p and in 4.7% of cases with an abnormal karyotype not involving chr17. Our results suggest that applying FISH of 17p13 to identify the number of copies of the TP53 gene could be beneficial in patients with a complex karyotype. We recommend using FISH of 17p13 in young patients with a normal karyotype or non-informative cytogenetics, and always in isolated del(17p)

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