Characterization and stabilization of GluLm and its application to deglycosylate dietary flavonoids and lignans

12 Pág.

Detalles Bibliográficos
Autores: Curiel, José Antonio, Ruiz de la Bastida, Ana, Langa, Susana, Peirotén, Ángela, Landete, José María
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2024
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/346588
Acceso en línea:http://hdl.handle.net/10261/346588
https://api.elsevier.com/content/abstract/scopus_id/85181584570
Access Level:acceso abierto
Palabra clave:GH3 glucosidase
Isoflavones
Lignans
Limosilactobacillus mucosae
Soy beverage
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spelling Characterization and stabilization of GluLm and its application to deglycosylate dietary flavonoids and lignansCuriel, José AntonioRuiz de la Bastida, AnaLanga, SusanaPeirotén, ÁngelaLandete, José MaríaGH3 glucosidaseIsoflavonesLignansLimosilactobacillus mucosaeSoy beverage12 Pág.This study describes the characterization of the recombinant GH3 aryl-β-glucosidase "GluLm" from Limosilactobacillus mucosae INIA P508, followed by its immobilization on an agarose support with the aim of developing an efficient application to increase the availability and concentration of flavonoid and lignan aglycones in a vegetal beverage. In previous studies, heterologous GluLm-producing strains demonstrated a great capacity to deglycosylate flavonoids. Nevertheless, the physicochemical properties and substrate spectrum of the enzyme remained unknown up to now. A high production of purified GluLm was achieved (14 mg L-1). GluLm exhibited optimal activity at broad ranges of pH (5.0-8.0) and temperature (25-60°C), as well as high affinity (Km of 0.10 mmol L-1) and specific constant (86554.0 mmol L-1 s-1) against p-nitrophenyl-β-D-glucopyranoside. Similar to other GH3 β-glucosidases described in lactic acid bacteria, GluLm exhibited β-xylosidase, β-galactosidase, and β-fucosidase activities. However, this study has revealed for the first time that a GH3 β-glucosidase is capable to hydrolyze different families of glycosylated phenolics such as flavonoids and secoiridoids. Although it exhibited low thermal stability, immobilization of GluLm improved its thermostability and allowed the development of a beverage based on soybeans and flaxseed extract with high concentration of bioactive isoflavone (daidzein, genistein), lignan (secoisolariciresinol, pinoresinol, and matairesinol), and other flavonoid aglycones. KEY POINTS: • Limosilactobacillus mucosae INIA P508 GluLm was purified and biochemically characterized • Immobilized GluLm efficiently deglycosylated flavonoids and lignans from a vegetal beverage • A viable application to produce vegetal beverages with a high content of aglycones is described.Open Access funding provided thanks to the CRUE-CSIC agreement with Springer Nature. This research was funded by the Spanish Ministry of Science and Innovation grants PID2020-119630RB-I00, RYC2019-026368-I, and by CSIC grant 202240I177 belonging to “Ayudas de Incorporación a Científicos Titulares.” A.R.d.l.B. is recipient of a pre-doctoral contract (PRE2018-086293), both from the Spanish Ministry of Science and Innovation.Peer reviewedSpringerConferencia de Rectores de las Universidades EspañolasConsejo Superior de Investigaciones Científicas (España)Ministerio de Ciencia e Innovación (España)Agencia Estatal de Investigación (España)Curiel, José Antonio [0000-0002-0111-1437]Ruiz de la Bastida, Ana [0000-0002-0650-7326]Langa, Susana [0000-0003-2729-219X]Peirotén, Ángela [0000-0002-1532-8530]Landete, José María [0000-0002-5147-3989]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202420242024info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/346588https://api.elsevier.com/content/abstract/scopus_id/85181584570reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-119630RB-I00info:eu-repo/grantAgreement/AEI/RYC2019-026368-I/info:eu-repo/grantAgreement/AEI/202240I177/Departamento de Tecnología de los alimentoshttps://doi.org/10.1007/s00253-023-12956-9Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3465882026-05-22T06:33:51Z
dc.title.none.fl_str_mv Characterization and stabilization of GluLm and its application to deglycosylate dietary flavonoids and lignans
title Characterization and stabilization of GluLm and its application to deglycosylate dietary flavonoids and lignans
spellingShingle Characterization and stabilization of GluLm and its application to deglycosylate dietary flavonoids and lignans
Curiel, José Antonio
GH3 glucosidase
Isoflavones
Lignans
Limosilactobacillus mucosae
Soy beverage
title_short Characterization and stabilization of GluLm and its application to deglycosylate dietary flavonoids and lignans
title_full Characterization and stabilization of GluLm and its application to deglycosylate dietary flavonoids and lignans
title_fullStr Characterization and stabilization of GluLm and its application to deglycosylate dietary flavonoids and lignans
title_full_unstemmed Characterization and stabilization of GluLm and its application to deglycosylate dietary flavonoids and lignans
title_sort Characterization and stabilization of GluLm and its application to deglycosylate dietary flavonoids and lignans
dc.creator.none.fl_str_mv Curiel, José Antonio
Ruiz de la Bastida, Ana
Langa, Susana
Peirotén, Ángela
Landete, José María
author Curiel, José Antonio
author_facet Curiel, José Antonio
Ruiz de la Bastida, Ana
Langa, Susana
Peirotén, Ángela
Landete, José María
author_role author
author2 Ruiz de la Bastida, Ana
Langa, Susana
Peirotén, Ángela
Landete, José María
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Conferencia de Rectores de las Universidades Españolas
Consejo Superior de Investigaciones Científicas (España)
Ministerio de Ciencia e Innovación (España)
Agencia Estatal de Investigación (España)
Curiel, José Antonio [0000-0002-0111-1437]
Ruiz de la Bastida, Ana [0000-0002-0650-7326]
Langa, Susana [0000-0003-2729-219X]
Peirotén, Ángela [0000-0002-1532-8530]
Landete, José María [0000-0002-5147-3989]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv GH3 glucosidase
Isoflavones
Lignans
Limosilactobacillus mucosae
Soy beverage
topic GH3 glucosidase
Isoflavones
Lignans
Limosilactobacillus mucosae
Soy beverage
description 12 Pág.
publishDate 2024
dc.date.none.fl_str_mv 2024
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/346588
https://api.elsevier.com/content/abstract/scopus_id/85181584570
url http://hdl.handle.net/10261/346588
https://api.elsevier.com/content/abstract/scopus_id/85181584570
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-119630RB-I00
info:eu-repo/grantAgreement/AEI/RYC2019-026368-I/
info:eu-repo/grantAgreement/AEI/202240I177/
Departamento de Tecnología de los alimentos
https://doi.org/10.1007/s00253-023-12956-9
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
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