High throughput stationary 13C-Metabolic Flux Analysis of 3-hydroxypropionic acid producing Pichia pastoris strains. Supplementary materials of Chapter 5 of the PhD Thesis of Albert Fina Romero

Supplementary files of Chapter 5 of the PhD Thesis of Albert Fina Romero. This chapter describes the fluxome of 3-hydroxypropionic acid producing Pichia pastoris recombinant strains. Glycerol was used as a sole carbon source. A High Throughput platform was used to simultaneously characterize the flu...

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Authors: Fina Romero, Albert|||0000-0001-7949-0484, Albiol i Sala, Joan|||0000-0001-5626-429X, Ferrer, Pau|||0000-0002-5287-4127
Format: conjunto de datos
Publication Date:2022
Country:España
Institution:Universitat Autònoma de Barcelona
Repository:Dipòsit Digital de Documents de la UAB
Language:English
OAI Identifier:oai:ddd.uab.cat:264385
Online Access:https://ddd.uab.cat/record/264385
https://dx.doi.org/urn:doi:10.5565/ddd.uab.cat/264385
Access Level:Open access
Keyword:Fluxomics
13C-Metabolic Flux Analysis (13C-MFA)
Pichia pastoris
High Throughput
Systems Biology
Ph
3-Hydroxypropionic acid
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spelling High throughput stationary 13C-Metabolic Flux Analysis of 3-hydroxypropionic acid producing Pichia pastoris strains. Supplementary materials of Chapter 5 of the PhD Thesis of Albert Fina RomeroFina Romero, Albert|||0000-0001-7949-0484Albiol i Sala, Joan|||0000-0001-5626-429XFerrer, Pau|||0000-0002-5287-4127Fluxomics13C-Metabolic Flux Analysis (13C-MFA)Pichia pastorisHigh ThroughputSystems BiologyPh3-Hydroxypropionic acidSupplementary files of Chapter 5 of the PhD Thesis of Albert Fina Romero. This chapter describes the fluxome of 3-hydroxypropionic acid producing Pichia pastoris recombinant strains. Glycerol was used as a sole carbon source. A High Throughput platform was used to simultaneously characterize the fluxome of 10 recombinant strains at pH 5 and 6 recombinant strains at pH 3.5. All the experiments were carried in triplicates. To determine the intracellular fluxes, stationary 13C-Metabolic Flux Analysis was used using the data of the isotopologue distribution of the proteinogenic amino acids. The files attached herein contain: the description of how to obtain the models used for this study, the raw data obtained from the mini-bioreactor cultivations, the files to process the raw data, and the results (bioprocess parameters and fluxes from 13C-Metabolic flux analysis and Flux Balance Analysis). 22022-01-0120222022-01-01Conjunt de dadeshttp://purl.org/coar/resource_type/c_ddb1VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/datasetapplication/ziptext/plainhttps://ddd.uab.cat/record/264385https://dx.doi.org/urn:doi:10.5565/ddd.uab.cat/264385reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengAgencia Estatal de Investigación https://doi.org/10.13039/501100011033 PID2019-104666GB-I00Ministerio de Ciencia e Innovación https://doi.org/10.13039/501100004837 FPU17/05434open accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons en que s'entrega l'obra al domini públic. Podeu copiar, modificar, distribuir l'obra i fer-ne comunicació pública, fins i tot amb finalitat comercial, sense demanar cap mena de permís.https://creativecommons.org/publicdomain/zero/1.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:2643852026-06-06T12:50:31Z
dc.title.none.fl_str_mv High throughput stationary 13C-Metabolic Flux Analysis of 3-hydroxypropionic acid producing Pichia pastoris strains. Supplementary materials of Chapter 5 of the PhD Thesis of Albert Fina Romero
title High throughput stationary 13C-Metabolic Flux Analysis of 3-hydroxypropionic acid producing Pichia pastoris strains. Supplementary materials of Chapter 5 of the PhD Thesis of Albert Fina Romero
spellingShingle High throughput stationary 13C-Metabolic Flux Analysis of 3-hydroxypropionic acid producing Pichia pastoris strains. Supplementary materials of Chapter 5 of the PhD Thesis of Albert Fina Romero
Fina Romero, Albert|||0000-0001-7949-0484
Fluxomics
13C-Metabolic Flux Analysis (13C-MFA)
Pichia pastoris
High Throughput
Systems Biology
Ph
3-Hydroxypropionic acid
title_short High throughput stationary 13C-Metabolic Flux Analysis of 3-hydroxypropionic acid producing Pichia pastoris strains. Supplementary materials of Chapter 5 of the PhD Thesis of Albert Fina Romero
title_full High throughput stationary 13C-Metabolic Flux Analysis of 3-hydroxypropionic acid producing Pichia pastoris strains. Supplementary materials of Chapter 5 of the PhD Thesis of Albert Fina Romero
title_fullStr High throughput stationary 13C-Metabolic Flux Analysis of 3-hydroxypropionic acid producing Pichia pastoris strains. Supplementary materials of Chapter 5 of the PhD Thesis of Albert Fina Romero
title_full_unstemmed High throughput stationary 13C-Metabolic Flux Analysis of 3-hydroxypropionic acid producing Pichia pastoris strains. Supplementary materials of Chapter 5 of the PhD Thesis of Albert Fina Romero
title_sort High throughput stationary 13C-Metabolic Flux Analysis of 3-hydroxypropionic acid producing Pichia pastoris strains. Supplementary materials of Chapter 5 of the PhD Thesis of Albert Fina Romero
dc.creator.none.fl_str_mv Fina Romero, Albert|||0000-0001-7949-0484
Albiol i Sala, Joan|||0000-0001-5626-429X
Ferrer, Pau|||0000-0002-5287-4127
author Fina Romero, Albert|||0000-0001-7949-0484
author_facet Fina Romero, Albert|||0000-0001-7949-0484
Albiol i Sala, Joan|||0000-0001-5626-429X
Ferrer, Pau|||0000-0002-5287-4127
author_role author
author2 Albiol i Sala, Joan|||0000-0001-5626-429X
Ferrer, Pau|||0000-0002-5287-4127
author2_role author
author
dc.subject.none.fl_str_mv Fluxomics
13C-Metabolic Flux Analysis (13C-MFA)
Pichia pastoris
High Throughput
Systems Biology
Ph
3-Hydroxypropionic acid
topic Fluxomics
13C-Metabolic Flux Analysis (13C-MFA)
Pichia pastoris
High Throughput
Systems Biology
Ph
3-Hydroxypropionic acid
description Supplementary files of Chapter 5 of the PhD Thesis of Albert Fina Romero. This chapter describes the fluxome of 3-hydroxypropionic acid producing Pichia pastoris recombinant strains. Glycerol was used as a sole carbon source. A High Throughput platform was used to simultaneously characterize the fluxome of 10 recombinant strains at pH 5 and 6 recombinant strains at pH 3.5. All the experiments were carried in triplicates. To determine the intracellular fluxes, stationary 13C-Metabolic Flux Analysis was used using the data of the isotopologue distribution of the proteinogenic amino acids. The files attached herein contain: the description of how to obtain the models used for this study, the raw data obtained from the mini-bioreactor cultivations, the files to process the raw data, and the results (bioprocess parameters and fluxes from 13C-Metabolic flux analysis and Flux Balance Analysis).
publishDate 2022
dc.date.none.fl_str_mv 2
2022-01-01
2022
2022-01-01
dc.type.none.fl_str_mv Conjunt de dades
http://purl.org/coar/resource_type/c_ddb1
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/dataset
format dataset
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/264385
https://dx.doi.org/urn:doi:10.5565/ddd.uab.cat/264385
url https://ddd.uab.cat/record/264385
https://dx.doi.org/urn:doi:10.5565/ddd.uab.cat/264385
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 PID2019-104666GB-I00
Ministerio de Ciencia e Innovación https://doi.org/10.13039/501100004837 FPU17/05434
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
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dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
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eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/zip
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dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
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