High throughput stationary 13C-Metabolic Flux Analysis of 3-hydroxypropionic acid producing Pichia pastoris strains. Supplementary materials of Chapter 5 of the PhD Thesis of Albert Fina Romero
Supplementary files of Chapter 5 of the PhD Thesis of Albert Fina Romero. This chapter describes the fluxome of 3-hydroxypropionic acid producing Pichia pastoris recombinant strains. Glycerol was used as a sole carbon source. A High Throughput platform was used to simultaneously characterize the flu...
| Autores: | , , |
|---|---|
| Tipo de recurso: | conjunto de datos |
| Fecha de publicación: | 2022 |
| País: | España |
| Institución: | Universitat Autònoma de Barcelona |
| Repositorio: | Dipòsit Digital de Documents de la UAB |
| Idioma: | inglés |
| OAI Identifier: | oai:ddd.uab.cat:264385 |
| Acceso en línea: | https://ddd.uab.cat/record/264385 https://dx.doi.org/urn:doi:10.5565/ddd.uab.cat/264385 |
| Access Level: | acceso abierto |
| Palabra clave: | Fluxomics 13C-Metabolic Flux Analysis (13C-MFA) Pichia pastoris High Throughput Systems Biology Ph 3-Hydroxypropionic acid |
| Sumario: | Supplementary files of Chapter 5 of the PhD Thesis of Albert Fina Romero. This chapter describes the fluxome of 3-hydroxypropionic acid producing Pichia pastoris recombinant strains. Glycerol was used as a sole carbon source. A High Throughput platform was used to simultaneously characterize the fluxome of 10 recombinant strains at pH 5 and 6 recombinant strains at pH 3.5. All the experiments were carried in triplicates. To determine the intracellular fluxes, stationary 13C-Metabolic Flux Analysis was used using the data of the isotopologue distribution of the proteinogenic amino acids. The files attached herein contain: the description of how to obtain the models used for this study, the raw data obtained from the mini-bioreactor cultivations, the files to process the raw data, and the results (bioprocess parameters and fluxes from 13C-Metabolic flux analysis and Flux Balance Analysis). |
|---|