Supplementary materials of research article "High throughput 13C-metabolic flux analysis of 3-hydroxypropionic acid producing Pichia pastoris reveals limited availability of acetyl-CoA and ATP due to tight control of the glycolytic flux"
Supplementary files of research article "High throughput 13C-metabolic flux analysis of 3-hydroxypropionic acid producing Pichia pastoris reveals limited availability of acetyl-CoA and ATP due to tight control of the glycolytic flux ". This research paper describes the fluxome of 3-hydroxy...
| Autores: | , , , , |
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| Formato: | conjunto de datos |
| Fecha de publicación: | 2023 |
| País: | España |
| Recursos: | Consorci de Serveis Universitaris de Catalunya (CSUC) |
| Repositorio: | CORA.Repositori de Dades de Recerca |
| OAI Identifier: | oai:dnet:cora.rdr____::96fb24369945f053825246dfc543ffbe |
| Acesso em linha: | https://doi.org/10.34810/DATA673 |
| Access Level: | acceso abierto |
| Palavra-chave: | Chemistry Engineering Fluxomics 13C-Metabolic Flux Analysis 13C-MFA Pichia pastoris High Throughput 3-hydroxypropionic acid pH |
| Resumo: | Supplementary files of research article "High throughput 13C-metabolic flux analysis of 3-hydroxypropionic acid producing Pichia pastoris reveals limited availability of acetyl-CoA and ATP due to tight control of the glycolytic flux ". This research paper describes the fluxome of 3-hydroxypropionic acid producing Pichia pastoris recombinant strains. Glycerol was used as a sole carbon source. A High Throughput platform was used to simultaneously characterize the fluxome of 10 recombinant strains at pH 5 and 6 recombinant strains at pH 3.5. All the experiments were carried in triplicates. To determine the intracellular fluxes, stationary 13C-Metabolic Flux Analysis was used using the data of the isotopologue distribution of the proteinogenic amino acids. The files attached herein contain: the description of how to obtain the models used for this study, the raw data obtained from the mini-bioreactor cultivations, the files to process the raw data, and the results (bioprocess parameters and fluxes from 13C-Metabolic flux analysis and Flux Balance Analysis). |
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