SPIM-fluid: open source light-sheet based platform for high-throughput imaging

Light sheet fluorescence microscopy has recently emerged as the technique of choice for obtaining high quality 3D images of whole organisms/embryos with low photodamage and fast acquisition rates. Here we present an open source unified implementation based on Arduino and Micromanager, which is capab...

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Detalles Bibliográficos
Autores: Gualda Manzano, Emilio José|||0000-0001-5948-5493, Pereira, Hugo, Vale, Tiago, Falcao Estrada, Marta, Brito, Catarina
Tipo de recurso: artículo
Fecha de publicación:2015
País:España
Institución:Universitat Politècnica de Catalunya (UPC)
Repositorio:UPCommons. Portal del coneixement obert de la UPC
Idioma:inglés
OAI Identifier:oai:upcommons.upc.edu:2117/346091
Acceso en línea:https://hdl.handle.net/2117/346091
https://dx.doi.org/10.1364/boe.6.004447
Access Level:acceso abierto
Palabra clave:Fluorescence microscopy
Microscòpia de fluorescència
Àrees temàtiques de la UPC::Ciències de la visió::Òptica física::Llum
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spelling SPIM-fluid: open source light-sheet based platform for high-throughput imagingGualda Manzano, Emilio José|||0000-0001-5948-5493Pereira, HugoVale, TiagoFalcao Estrada, MartaBrito, CatarinaFluorescence microscopyFluorescence microscopyMicroscòpia de fluorescènciaÀrees temàtiques de la UPC::Ciències de la visió::Òptica física::LlumLight sheet fluorescence microscopy has recently emerged as the technique of choice for obtaining high quality 3D images of whole organisms/embryos with low photodamage and fast acquisition rates. Here we present an open source unified implementation based on Arduino and Micromanager, which is capable of operating Light Sheet Microscopes for automatized 3D high-throughput imaging on three-dimensional cell cultures and model organisms like zebrafish, oriented to massive drug screening20152015-11-0120212021-05-26journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/2117/346091https://dx.doi.org/10.1364/boe.6.004447reponame:UPCommons. Portal del coneixement obert de la UPCinstname:Universitat Politècnica de Catalunya (UPC)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivs 3.0 Spainhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/info:eu-repo/semantics/openAccessoai:upcommons.upc.edu:2117/3460912026-05-27T15:37:01Z
dc.title.none.fl_str_mv SPIM-fluid: open source light-sheet based platform for high-throughput imaging
title SPIM-fluid: open source light-sheet based platform for high-throughput imaging
spellingShingle SPIM-fluid: open source light-sheet based platform for high-throughput imaging
Gualda Manzano, Emilio José|||0000-0001-5948-5493
Fluorescence microscopy
Fluorescence microscopy
Microscòpia de fluorescència
Àrees temàtiques de la UPC::Ciències de la visió::Òptica física::Llum
title_short SPIM-fluid: open source light-sheet based platform for high-throughput imaging
title_full SPIM-fluid: open source light-sheet based platform for high-throughput imaging
title_fullStr SPIM-fluid: open source light-sheet based platform for high-throughput imaging
title_full_unstemmed SPIM-fluid: open source light-sheet based platform for high-throughput imaging
title_sort SPIM-fluid: open source light-sheet based platform for high-throughput imaging
dc.creator.none.fl_str_mv Gualda Manzano, Emilio José|||0000-0001-5948-5493
Pereira, Hugo
Vale, Tiago
Falcao Estrada, Marta
Brito, Catarina
author Gualda Manzano, Emilio José|||0000-0001-5948-5493
author_facet Gualda Manzano, Emilio José|||0000-0001-5948-5493
Pereira, Hugo
Vale, Tiago
Falcao Estrada, Marta
Brito, Catarina
author_role author
author2 Pereira, Hugo
Vale, Tiago
Falcao Estrada, Marta
Brito, Catarina
author2_role author
author
author
author
dc.subject.none.fl_str_mv Fluorescence microscopy
Fluorescence microscopy
Microscòpia de fluorescència
Àrees temàtiques de la UPC::Ciències de la visió::Òptica física::Llum
topic Fluorescence microscopy
Fluorescence microscopy
Microscòpia de fluorescència
Àrees temàtiques de la UPC::Ciències de la visió::Òptica física::Llum
description Light sheet fluorescence microscopy has recently emerged as the technique of choice for obtaining high quality 3D images of whole organisms/embryos with low photodamage and fast acquisition rates. Here we present an open source unified implementation based on Arduino and Micromanager, which is capable of operating Light Sheet Microscopes for automatized 3D high-throughput imaging on three-dimensional cell cultures and model organisms like zebrafish, oriented to massive drug screening
publishDate 2015
dc.date.none.fl_str_mv 2015
2015-11-01
2021
2021-05-26
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/2117/346091
https://dx.doi.org/10.1364/boe.6.004447
url https://hdl.handle.net/2117/346091
https://dx.doi.org/10.1364/boe.6.004447
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivs 3.0 Spain
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivs 3.0 Spain
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:UPCommons. Portal del coneixement obert de la UPC
instname:Universitat Politècnica de Catalunya (UPC)
instname_str Universitat Politècnica de Catalunya (UPC)
reponame_str UPCommons. Portal del coneixement obert de la UPC
collection UPCommons. Portal del coneixement obert de la UPC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,301629

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