SPIM-fluid: open source light-sheet based platform for high-throughput imaging
Light sheet fluorescence microscopy has recently emerged as the technique of choice for obtaining high quality 3D images of whole organisms/embryos with low photodamage and fast acquisition rates. Here we present an open source unified implementation based on Arduino and Micromanager, which is capab...
| Autores: | , , , , |
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| Tipo de recurso: | artículo |
| Fecha de publicación: | 2015 |
| País: | España |
| Institución: | Universitat Politècnica de Catalunya (UPC) |
| Repositorio: | UPCommons. Portal del coneixement obert de la UPC |
| Idioma: | inglés |
| OAI Identifier: | oai:upcommons.upc.edu:2117/346091 |
| Acceso en línea: | https://hdl.handle.net/2117/346091 https://dx.doi.org/10.1364/boe.6.004447 |
| Access Level: | acceso abierto |
| Palabra clave: | Fluorescence microscopy Microscòpia de fluorescència Àrees temàtiques de la UPC::Ciències de la visió::Òptica física::Llum |
| Sumario: | Light sheet fluorescence microscopy has recently emerged as the technique of choice for obtaining high quality 3D images of whole organisms/embryos with low photodamage and fast acquisition rates. Here we present an open source unified implementation based on Arduino and Micromanager, which is capable of operating Light Sheet Microscopes for automatized 3D high-throughput imaging on three-dimensional cell cultures and model organisms like zebrafish, oriented to massive drug screening |
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