SPIM-fluid: open source light-sheet based platform for high-throughput imaging
Light sheet fluorescence microscopy has recently emerged as the technique of choice for obtaining high quality 3D images of whole organisms/embryos with low photodamage and fast acquisition rates. Here we present an open source unified implementation based on Arduino and Micromanager, which is capab...
| Authors: | , , , , |
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| Format: | article |
| Publication Date: | 2015 |
| Country: | España |
| Institution: | Universitat Politècnica de Catalunya (UPC) |
| Repository: | UPCommons. Portal del coneixement obert de la UPC |
| Language: | English |
| OAI Identifier: | oai:upcommons.upc.edu:2117/346091 |
| Online Access: | https://hdl.handle.net/2117/346091 https://dx.doi.org/10.1364/boe.6.004447 |
| Access Level: | Open access |
| Keyword: | Fluorescence microscopy Microscòpia de fluorescència Àrees temàtiques de la UPC::Ciències de la visió::Òptica física::Llum |
| Summary: | Light sheet fluorescence microscopy has recently emerged as the technique of choice for obtaining high quality 3D images of whole organisms/embryos with low photodamage and fast acquisition rates. Here we present an open source unified implementation based on Arduino and Micromanager, which is capable of operating Light Sheet Microscopes for automatized 3D high-throughput imaging on three-dimensional cell cultures and model organisms like zebrafish, oriented to massive drug screening |
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