Imaging of human differentiated 3D neural aggregates using light sheet fluorescence microscopy
The development of three dimensional (3D) cell cultures represents a big step for the better understanding of cell behavior and disease in a more natural like environment, providing not only single but multiple cell type interactions in a complex 3D matrix, highly resembling physiological conditions...
| Autores: | , , , , |
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| Tipo de recurso: | artículo |
| Fecha de publicación: | 2014 |
| País: | España |
| Institución: | Universitat Politècnica de Catalunya (UPC) |
| Repositorio: | UPCommons. Portal del coneixement obert de la UPC |
| Idioma: | inglés |
| OAI Identifier: | oai:upcommons.upc.edu:2117/344024 |
| Acceso en línea: | https://hdl.handle.net/2117/344024 https://dx.doi.org/10.3389/fncel.2014.00221 |
| Access Level: | acceso abierto |
| Palabra clave: | Microscopy, Fluorescence Fluorescence microscopy Light-sheet fluorescence microscopy 3D neural aggregates Calcium imaging Live/dead assays Microscòpia de fluorescència Àrees temàtiques de la UPC::Ciències de la salut::Medicina::Neurologia |
| Sumario: | The development of three dimensional (3D) cell cultures represents a big step for the better understanding of cell behavior and disease in a more natural like environment, providing not only single but multiple cell type interactions in a complex 3D matrix, highly resembling physiological conditions. Light sheet fluorescence microscopy (LSFM) is becoming an excellent tool for fast imaging of such 3D biological structures. We demonstrate the potential of this technique for the imaging of human differentiated 3D neural aggregates in fixed and live samples, namely calcium imaging and cell death processes, showing the power of imaging modality compared with traditional microscopy. |
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