Visualization and quantification of the cellular and extracellular components of Salmonella Agona biofilms at different stages of development

1). Riesco Peláez, Félix: Department of Electrical Engineering and Systems Engineering and Automatic Control, School of Industrial, Computer and Aerospace Engineering, University of León, E-24071, León, Spain. 2) MATERIAS: Tecnología de los Alimentos, Informática (software)

Detalles Bibliográficos
Autores: González Machado, María del Camino, Capita González, Rosa María, Riesco Peláez, Félix, Alonso Calleja, Carlos
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2018
País:España
Institución:Universidad de León
Repositorio:BULERIA. Repositorio Institucional de la Universidad de León
OAI Identifier:oai:buleria.unileon.es:10612/24325
Acceso en línea:https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0200011
https://hdl.handle.net/10612/24325
https://doi.org/10.1371/journal.pone.0200011
Access Level:acceso abierto
Palabra clave:Tecnología de los alimentos
Bacterial biofilms
Expolysaccharides
Confocal laser microscopy
Polystyrene
Lipids
Salmonella enterica
FORM BIOFILMS
ABILITY
ARCHITECTURE
VIABILITY
BIOCIDES
STRAINS
CLSM
LASER-SCANNING MICROSCOPY
BioRCA 1.7 software
3206.05 Agentes Patógenos de Los Alimentos
3309 Tecnología de Los Alimentos
3309.15 Higiene de Los Alimentos
3309.90 Microbiología de Alimentos
1203.17 Informática (programas)
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spelling Visualization and quantification of the cellular and extracellular components of Salmonella Agona biofilms at different stages of developmentGonzález Machado, María del CaminoCapita González, Rosa MaríaRiesco Peláez, FélixAlonso Calleja, CarlosTecnología de los alimentosBacterial biofilmsExpolysaccharidesConfocal laser microscopyPolystyreneLipidsSalmonella entericaFORM BIOFILMSABILITYARCHITECTUREVIABILITYBIOCIDESSTRAINSCLSMLASER-SCANNING MICROSCOPYBioRCA 1.7 software3206.05 Agentes Patógenos de Los Alimentos3309 Tecnología de Los Alimentos3309.15 Higiene de Los Alimentos3309.90 Microbiología de Alimentos1203.17 Informática (programas)1). Riesco Peláez, Félix: Department of Electrical Engineering and Systems Engineering and Automatic Control, School of Industrial, Computer and Aerospace Engineering, University of León, E-24071, León, Spain. 2) MATERIAS: Tecnología de los Alimentos, Informática (software)[EN]. Salmonella is a major food-borne pathogen able to persist in food processing environments because of its ability to form biofilms. A Salmonella enterica serotype Agona isolate from poultry (S24) was grown at 37C in biofilms for up to 144 hours (H144) in attachment to polystyrene surfaces. Biofilm structures were examined at different stages in their development (H3, H24, H48, H72, H96 and H144) using confocal laser scanning microscopy (CLSM) in conjunction with fluorescent dyes for live cells (SYTO 9), dead cells (propidium iodide), proteins (fluorescein isothiocyanate isomer I), lipids (DiD’oil), α-polysaccharides (concanavalin A, tetramethylrhodamine conjugate), and β-polysaccharides (calcofluor white M2R). Strain S24 developed a robust biofilm at H72 (biovolume of 166,852.5 ± 13,681.8 μm3 in the observation field of 16,078.2 μm2). The largest biovolume of live cells was also detected at H72 (128,110.3 ± 4,969.1 μm3), decreasing thereafter, which was probably owing to the detachment of cells prior to a new phase of colonization. The percentage of dead cells with regard to total cells in the biofilms increased throughout the incubation, ranging from 2.3 ± 1.1% (H24) to 44.2 ± 11.0% (H144). Proteins showed the greatest biovolume among the extracellular components within the biofilms, with values ranging from 1,295.1 ± 1,294.9 μm3 (H3) to 19,186.2 ± 8,536.0 μm3 (H96). Maximum biovolume values of 15,171.9 ± 660.7 μm3 (H48), 7,055.3 ± 4,415.2 μm3 (H144), and 2,548.6 ± 1,597.5 μm3 (H72) were observed for β-polysaccharides, α-polysaccharides and lipids, respectively. A strong (P < 0.01) positive correlation was found between the total biovolume of biofilm and the biovolume of live cells, proteins and β-polysaccharides, which may serve as useful markers of biofilm formation. The present work provides new insights into the formation of S. Agona biofilms. Our findings may contribute to the designing of reliable strategies for preventing and removing these bacterial communities.SIThis research was supported by the Spanish Ministry of Economy, Industry and Competitiveness (INIA; RTA2014-00045- C03-02). Camino González-Machado was beneficiary of a fellowship of the Regional Government of Castile and Leon (Operational Programme for Youth Employment, European Social Fund and Youth Employment Initiative).Public Library Science, San Francisco (USA)Nutricion y BromatologiaFacultad de Veterinaria2018info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0200011https://hdl.handle.net/10612/24325https://doi.org/10.1371/journal.pone.0200011reponame:BULERIA. Repositorio Institucional de la Universidad de Leóninstname:Universidad de LeónInglésinfo:eu-repo/grantAgreement/MINECO/ Programa Estatal de I+D+I Orientada a los Retos de la Sociedad / RTA2014-00045-C03-02 http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:buleria.unileon.es:10612/243252026-06-24T12:43:27Z
dc.title.none.fl_str_mv Visualization and quantification of the cellular and extracellular components of Salmonella Agona biofilms at different stages of development
title Visualization and quantification of the cellular and extracellular components of Salmonella Agona biofilms at different stages of development
spellingShingle Visualization and quantification of the cellular and extracellular components of Salmonella Agona biofilms at different stages of development
González Machado, María del Camino
Tecnología de los alimentos
Bacterial biofilms
Expolysaccharides
Confocal laser microscopy
Polystyrene
Lipids
Salmonella enterica
FORM BIOFILMS
ABILITY
ARCHITECTURE
VIABILITY
BIOCIDES
STRAINS
CLSM
LASER-SCANNING MICROSCOPY
BioRCA 1.7 software
3206.05 Agentes Patógenos de Los Alimentos
3309 Tecnología de Los Alimentos
3309.15 Higiene de Los Alimentos
3309.90 Microbiología de Alimentos
1203.17 Informática (programas)
title_short Visualization and quantification of the cellular and extracellular components of Salmonella Agona biofilms at different stages of development
title_full Visualization and quantification of the cellular and extracellular components of Salmonella Agona biofilms at different stages of development
title_fullStr Visualization and quantification of the cellular and extracellular components of Salmonella Agona biofilms at different stages of development
title_full_unstemmed Visualization and quantification of the cellular and extracellular components of Salmonella Agona biofilms at different stages of development
title_sort Visualization and quantification of the cellular and extracellular components of Salmonella Agona biofilms at different stages of development
dc.creator.none.fl_str_mv González Machado, María del Camino
Capita González, Rosa María
Riesco Peláez, Félix
Alonso Calleja, Carlos
author González Machado, María del Camino
author_facet González Machado, María del Camino
Capita González, Rosa María
Riesco Peláez, Félix
Alonso Calleja, Carlos
author_role author
author2 Capita González, Rosa María
Riesco Peláez, Félix
Alonso Calleja, Carlos
author2_role author
author
author
dc.contributor.none.fl_str_mv Nutricion y Bromatologia
Facultad de Veterinaria
dc.subject.none.fl_str_mv Tecnología de los alimentos
Bacterial biofilms
Expolysaccharides
Confocal laser microscopy
Polystyrene
Lipids
Salmonella enterica
FORM BIOFILMS
ABILITY
ARCHITECTURE
VIABILITY
BIOCIDES
STRAINS
CLSM

LASER-SCANNING MICROSCOPY
BioRCA 1.7 software
3206.05 Agentes Patógenos de Los Alimentos
3309 Tecnología de Los Alimentos
3309.15 Higiene de Los Alimentos
3309.90 Microbiología de Alimentos
1203.17 Informática (programas)
topic Tecnología de los alimentos
Bacterial biofilms
Expolysaccharides
Confocal laser microscopy
Polystyrene
Lipids
Salmonella enterica
FORM BIOFILMS
ABILITY
ARCHITECTURE
VIABILITY
BIOCIDES
STRAINS
CLSM
LASER-SCANNING MICROSCOPY
BioRCA 1.7 software
3206.05 Agentes Patógenos de Los Alimentos
3309 Tecnología de Los Alimentos
3309.15 Higiene de Los Alimentos
3309.90 Microbiología de Alimentos
1203.17 Informática (programas)
description 1). Riesco Peláez, Félix: Department of Electrical Engineering and Systems Engineering and Automatic Control, School of Industrial, Computer and Aerospace Engineering, University of León, E-24071, León, Spain. 2) MATERIAS: Tecnología de los Alimentos, Informática (software)
publishDate 2018
dc.date.none.fl_str_mv 2018
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0200011
https://hdl.handle.net/10612/24325
https://doi.org/10.1371/journal.pone.0200011
url https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0200011
https://hdl.handle.net/10612/24325
https://doi.org/10.1371/journal.pone.0200011
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/MINECO/ Programa Estatal de I+D+I Orientada a los Retos de la Sociedad / RTA2014-00045-C03-02
dc.rights.none.fl_str_mv http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library Science, San Francisco (USA)
publisher.none.fl_str_mv Public Library Science, San Francisco (USA)
dc.source.none.fl_str_mv reponame:BULERIA. Repositorio Institucional de la Universidad de León
instname:Universidad de León
instname_str Universidad de León
reponame_str BULERIA. Repositorio Institucional de la Universidad de León
collection BULERIA. Repositorio Institucional de la Universidad de León
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.811543

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