Molecular gated nanoporous anodic alumina for the detection of cocaine

[EN] We present herein the use of nanoporous anodic alumina (NAA) as a suitable support to implement molecular gates for sensing applications. In our design, a NAA support is loaded with a fluorescent reporter (rhodamine B) and functionalized with a short single-stranded DNA. Then pores are blocked...

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Autores: Ribes, Àngela, Xifre Perez, Elisabet, Pardo Vicente, María Teresa, Marsal, Lluis F., Aznar, Elena|||0000-0003-0361-3876, Sancenón Galarza, Félix|||0000-0002-5205-7135, Martínez-Máñez, Ramón|||0000-0001-5873-9674
Tipo de recurso: artículo
Fecha de publicación:2016
País:España
Institución:Universitat Politècnica de València (UPV)
Repositorio:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
Idioma:inglés
OAI Identifier:oai:riunet.upv.es:10251/88178
Acceso en línea:https://riunet.upv.es/handle/10251/88178
Access Level:acceso abierto
Palabra clave:BIORESPONSIVE CONTROLLED-RELEASE
MESOPOROUS SILICA NANOPARTICLES
RESPONSIVE DRUG-DELIVERY
APTAMER-BASED SENSOR
ORAL FLUID
DNA
SYSTEMS
PLATFORM
SPECIFICITY
APTASENSOR
Electron Microscopy Service of the UPV
QUIMICA INORGANICA
QUIMICA ORGANICA
QUIMICA ANALITICA
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network_name_str España
repository_id_str
spelling Molecular gated nanoporous anodic alumina for the detection of cocaineRibes, ÀngelaXifre Perez, ElisabetPardo Vicente, María TeresaMarsal, Lluis F.Aznar, Elena|||0000-0003-0361-3876Sancenón Galarza, Félix|||0000-0002-5205-7135Martínez-Máñez, Ramón|||0000-0001-5873-9674BIORESPONSIVE CONTROLLED-RELEASEMESOPOROUS SILICA NANOPARTICLESRESPONSIVE DRUG-DELIVERYAPTAMER-BASED SENSORORAL FLUIDDNASYSTEMSPLATFORMSPECIFICITYAPTASENSORElectron Microscopy Service of the UPVQUIMICA INORGANICAQUIMICA ORGANICAQUIMICA ANALITICA[EN] We present herein the use of nanoporous anodic alumina (NAA) as a suitable support to implement molecular gates for sensing applications. In our design, a NAA support is loaded with a fluorescent reporter (rhodamine B) and functionalized with a short single-stranded DNA. Then pores are blocked by the subsequent hybridisation of a specific cocaine aptamer. The response of the gated material was studied in aqueous solution. In a typical experiment, the support was immersed in hybridisation buffer solution in the absence or presence of cocaine. At certain times, the release of rhodamine B from pore voids was measured by fluorescence spectroscopy. The capped NAA support showed poor cargo delivery, but presence of cocaine in the solution selectively induced rhodamine B release. By this simple procedure a limit of detection as low as 5 × 10−7 M was calculated for cocaine. The gated NAA was successfully applied to detect cocaine in saliva samples and the possible re-use of the nanostructures was assessed. Based on these results, we believe that NAA could be a suitable support to prepare optical gated probes with a synergic combination of the favourable features of selected gated sensing systems and NAA.We thank Projects MAT2015-64139-C4-1-R and TEC2015-71324-R (MINECO/FEDER), the Catalan Government (Project 2014 SGR 1344), the ICREA (ICREA2014 Academia Award) and the Generalitat Valenciana (Project PROMETEOII/2014/047) for support. We also thank to the Agencia Espanola del Medicamento y Productos Sanitarios for its concessions. A.R. thanks the UPV for her predoctoral fellowship. The authors also thank the Electron Microscopy Service at UPV for support.Nature Publishing GroupEscuela Técnica Superior de Ingeniería Aeroespacial y Diseño IndustrialDepartamento de QuímicaEscuela Técnica Superior de Ingeniería IndustrialInstituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo TecnológicoMinisterio de Economía y CompetitividadGeneralitat ValencianaGeneralitat de CatalunyaRepositorio Institucional de la Universitat Politècnica de València Riunet20162016-12-07journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://riunet.upv.es/handle/10251/88178reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valénciainstname:Universitat Politècnica de València (UPV)InglésengMinisterio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 MAT2015-64139-C4-1-R NANOMATERIALES INTELIGENTES, SONDAS Y DISPOSITIVOS PARA EL DESARROLLO INTEGRADO DE NUEVAS HERRAMIENTAS APLICADAS AL CAMPO BIOMEDICOGeneralitat de Catalunya Generalitat de Catalunya 2014 SGR 1344Generalitat Valenciana https://doi.org/10.13039/501100003359 PROMETEOII%2F2014%2F047 Nuevas aproximaciones para el diseño de materiales de liberación controlada y la detección de compuestos peligrososMinisterio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 TEC2015-71324-R DESARROLLO DE CELULAS SOLARES ORGANICAS FOTOVOLTAICAS Y BIOSENSORES FOTONICOS BASADOS EN MICRO -NANOESTRUCTURAS POROSAS Y POLIMEROS NANOESTRUCTURADOSopen accesshttp://purl.org/coar/access_right/c_abf2Reserva de todos los derechoshttp://rightsstatements.org/vocab/InC/1.0/info:eu-repo/semantics/openAccessoai:riunet.upv.es:10251/881782026-06-13T07:49:27Z
dc.title.none.fl_str_mv Molecular gated nanoporous anodic alumina for the detection of cocaine
title Molecular gated nanoporous anodic alumina for the detection of cocaine
spellingShingle Molecular gated nanoporous anodic alumina for the detection of cocaine
Ribes, Àngela
BIORESPONSIVE CONTROLLED-RELEASE
MESOPOROUS SILICA NANOPARTICLES
RESPONSIVE DRUG-DELIVERY
APTAMER-BASED SENSOR
ORAL FLUID
DNA
SYSTEMS
PLATFORM
SPECIFICITY
APTASENSOR
Electron Microscopy Service of the UPV
QUIMICA INORGANICA
QUIMICA ORGANICA
QUIMICA ANALITICA
title_short Molecular gated nanoporous anodic alumina for the detection of cocaine
title_full Molecular gated nanoporous anodic alumina for the detection of cocaine
title_fullStr Molecular gated nanoporous anodic alumina for the detection of cocaine
title_full_unstemmed Molecular gated nanoporous anodic alumina for the detection of cocaine
title_sort Molecular gated nanoporous anodic alumina for the detection of cocaine
dc.creator.none.fl_str_mv Ribes, Àngela
Xifre Perez, Elisabet
Pardo Vicente, María Teresa
Marsal, Lluis F.
Aznar, Elena|||0000-0003-0361-3876
Sancenón Galarza, Félix|||0000-0002-5205-7135
Martínez-Máñez, Ramón|||0000-0001-5873-9674
author Ribes, Àngela
author_facet Ribes, Àngela
Xifre Perez, Elisabet
Pardo Vicente, María Teresa
Marsal, Lluis F.
Aznar, Elena|||0000-0003-0361-3876
Sancenón Galarza, Félix|||0000-0002-5205-7135
Martínez-Máñez, Ramón|||0000-0001-5873-9674
author_role author
author2 Xifre Perez, Elisabet
Pardo Vicente, María Teresa
Marsal, Lluis F.
Aznar, Elena|||0000-0003-0361-3876
Sancenón Galarza, Félix|||0000-0002-5205-7135
Martínez-Máñez, Ramón|||0000-0001-5873-9674
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Escuela Técnica Superior de Ingeniería Aeroespacial y Diseño Industrial
Departamento de Química
Escuela Técnica Superior de Ingeniería Industrial
Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico
Ministerio de Economía y Competitividad
Generalitat Valenciana
Generalitat de Catalunya
Repositorio Institucional de la Universitat Politècnica de València Riunet
dc.subject.none.fl_str_mv BIORESPONSIVE CONTROLLED-RELEASE
MESOPOROUS SILICA NANOPARTICLES
RESPONSIVE DRUG-DELIVERY
APTAMER-BASED SENSOR
ORAL FLUID
DNA
SYSTEMS
PLATFORM
SPECIFICITY
APTASENSOR
Electron Microscopy Service of the UPV
QUIMICA INORGANICA
QUIMICA ORGANICA
QUIMICA ANALITICA
topic BIORESPONSIVE CONTROLLED-RELEASE
MESOPOROUS SILICA NANOPARTICLES
RESPONSIVE DRUG-DELIVERY
APTAMER-BASED SENSOR
ORAL FLUID
DNA
SYSTEMS
PLATFORM
SPECIFICITY
APTASENSOR
Electron Microscopy Service of the UPV
QUIMICA INORGANICA
QUIMICA ORGANICA
QUIMICA ANALITICA
description [EN] We present herein the use of nanoporous anodic alumina (NAA) as a suitable support to implement molecular gates for sensing applications. In our design, a NAA support is loaded with a fluorescent reporter (rhodamine B) and functionalized with a short single-stranded DNA. Then pores are blocked by the subsequent hybridisation of a specific cocaine aptamer. The response of the gated material was studied in aqueous solution. In a typical experiment, the support was immersed in hybridisation buffer solution in the absence or presence of cocaine. At certain times, the release of rhodamine B from pore voids was measured by fluorescence spectroscopy. The capped NAA support showed poor cargo delivery, but presence of cocaine in the solution selectively induced rhodamine B release. By this simple procedure a limit of detection as low as 5 × 10−7 M was calculated for cocaine. The gated NAA was successfully applied to detect cocaine in saliva samples and the possible re-use of the nanostructures was assessed. Based on these results, we believe that NAA could be a suitable support to prepare optical gated probes with a synergic combination of the favourable features of selected gated sensing systems and NAA.
publishDate 2016
dc.date.none.fl_str_mv 2016
2016-12-07
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://riunet.upv.es/handle/10251/88178
url https://riunet.upv.es/handle/10251/88178
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Ministerio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 MAT2015-64139-C4-1-R NANOMATERIALES INTELIGENTES, SONDAS Y DISPOSITIVOS PARA EL DESARROLLO INTEGRADO DE NUEVAS HERRAMIENTAS APLICADAS AL CAMPO BIOMEDICO
Generalitat de Catalunya Generalitat de Catalunya 2014 SGR 1344
Generalitat Valenciana https://doi.org/10.13039/501100003359 PROMETEOII%2F2014%2F047 Nuevas aproximaciones para el diseño de materiales de liberación controlada y la detección de compuestos peligrosos
Ministerio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 TEC2015-71324-R DESARROLLO DE CELULAS SOLARES ORGANICAS FOTOVOLTAICAS Y BIOSENSORES FOTONICOS BASADOS EN MICRO -NANOESTRUCTURAS POROSAS Y POLIMEROS NANOESTRUCTURADOS
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reserva de todos los derechos
http://rightsstatements.org/vocab/InC/1.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reserva de todos los derechos
http://rightsstatements.org/vocab/InC/1.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
instname:Universitat Politècnica de València (UPV)
instname_str Universitat Politècnica de València (UPV)
reponame_str RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
collection RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.301629

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