Avidin-gated mesoporous silica nanoparticles for signal amplification in electrochemical biosensor
[EN] We report herein a novel sensing strategy for electrochemical biosensors, by using mesoporous silica nanoparticles loaded with the redox probe methylene blue and capped with an avidin/imminobiotin stimulus-responsive gate-like ensemble as signal amplification element. As a proof of concept, an...
| Autores: | , , , , , |
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| Tipo de recurso: | artículo |
| Fecha de publicación: | 2019 |
| País: | España |
| Institución: | Universitat Politècnica de València (UPV) |
| Repositorio: | RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia |
| Idioma: | inglés |
| OAI Identifier: | oai:riunet.upv.es:10251/195816 |
| Acceso en línea: | https://riunet.upv.es/handle/10251/195816 |
| Access Level: | acceso abierto |
| Palabra clave: | Aptamer Biosensor Carcinoembryonic antigen Mesoporous silica nanoparticles Signal amplification QUIMICA INORGANICA |
| Sumario: | [EN] We report herein a novel sensing strategy for electrochemical biosensors, by using mesoporous silica nanoparticles loaded with the redox probe methylene blue and capped with an avidin/imminobiotin stimulus-responsive gate-like ensemble as signal amplification element. As a proof of concept, an aptasensor for carcinoembryonic antigen (CEA) was constructed by attaching a biotin and thiol-functionalized anti-CEA DNA hairpin aptamer on gold nanoparticles modified carbon screen-printed electrodes. The biosensing approach relied on the unfolding of the aptamer molecule after specific recognition of CEA, unmasking the biotin residue and allowing further association with the avidin-capped mesoporous nanocarrier. Incubation with H2SO4 trigger the release of the encapsulated redox probe allowing the detection of the cancer biomarker from 1.0 pg/mL to 160 ng/mL. |
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