Green method for quantification of gatifloxacin in eye drops using UV by eco-scale assessment

Gatifloxacin (GAT), a fluoroquinolone, does not present a monograph described in official compendia and the literature presents some analytical methods for its evaluation, but with space for eco-efficient methods. The objective was to develop and validate a green and indicative of stability method b...

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Detalles Bibliográficos
Autores: Teixeira, Max Well Silva, Gil, Eric de Souza, Kogawa, Ana
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2024
País:Brasil
Institución:Universidade Federal do Rio Grande do Sul (UFRGS)
Repositorio:Revista Drug Analytical Research
Idioma:inglés
OAI Identifier:oai:seer.ufrgs.br:article/134965
Acceso en línea:https://seer.ufrgs.br/index.php/dar/article/view/134965
Access Level:acceso abierto
Palabra clave:gatifloxacin
eye drops
spectrophotometry in ultraviolet region
green analytical chemistry
Descripción
Sumario:Gatifloxacin (GAT), a fluoroquinolone, does not present a monograph described in official compendia and the literature presents some analytical methods for its evaluation, but with space for eco-efficient methods. The objective was to develop and validate a green and indicative of stability method by UV for quantitative evaluation of GAT in eye drops. Purified water, as a diluent, quartz cubette and 286 nm were used. The method was linear in the range of 4 – 12 µg mL-1 (0.9997), precise (RSD < 5 %), selective in the comparison of standard and sample and by the forced degradation, exact with average recovery of 100.21 %, robust against changes in wavelength, source of purified water and use of ultrasound. The method was able to quantify and evaluate the stability of GAT in eye drops in green form according to the Eco-Scale Assessment.