EXCELLENT GREEN ANALYSIS FOR QUANTIFICATION OF GATIFLOXACIN IN EYE DROPS BY ECO-SCALE ASSESSMENT

Gatifloxacin (GAT), a fourth-generation fluoroquinolone, is currently marketed primarily in the form of eye drops. The literature shows physical-chemical methods with a predominance of the use of toxic organic solvents, which promote the generation of potentially harmful residues both for the enviro...

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Detalles Bibliográficos
Autores: Freire, Rafael Lima, Sinzervinch, Aline, Marreto, Ricardo Neves, Kogawa, Ana Carolina
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2025
País:Brasil
Institución:Pontifícia Universidade Católica de Goiás (PUC-GO)
Repositorio:Estudos (Goiânia. Online)
Idioma:inglés
OAI Identifier:oai:ojs2.seer.pucgoias.edu.br:article/14937
Acceso en línea:https://seer.pucgoias.edu.br/index.php/estudos/article/view/14937
Access Level:acceso abierto
Palabra clave:gatifloxacin
eye drops
spectrophotometry in visible region
green analytical chemistry
Eco-Scale Assessment
Colírio
Espectrofotometria na Região do Visível
Gatifloxacino
Química Analítica Verde
Descripción
Sumario:Gatifloxacin (GAT), a fourth-generation fluoroquinolone, is currently marketed primarily in the form of eye drops. The literature shows physical-chemical methods with a predominance of the use of toxic organic solvents, which promote the generation of potentially harmful residues both for the environment and for the operator. The objective was to develop and validate an excellent green method in the visible region for quantification of GAT in eye drops. Purified water and NaOH 0.1 M, as a diluent, and phenolphthalein 1 %, for reaction, at 552 nm were used. The method was linear in the range of 7-12 µg/mL (0.9996), precise (relative standard deviation < 5 %), selective through sample adjuvants, exact (mean recovery of 100.88 %) and robust against changes in wavelength and microplate usage. The content obtained with the proposed method was 98.21 % and is in agreement with the official compendium and other methods described in the literature. The method is a green proposal to quantify GAT in eye drops according to the Eco-Scale Assessment tool.