Thermal, chemical and photocatalytic inactivation of Lactobacillus plantarum bacteriophages
The effect of several biocides, thermal treatments, and photocatalysis on the viability of four Lactobacillus plantarum phages was investigated. Times to achieve 99% inactivation (T99) of phages at 63, 72, and 90°C were evaluated in four suspension media: deMan Rogosa Sharpe broth, reconstituted sk...
| Autores: | , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2009 |
| País: | Argentina |
| Institución: | Consejo Nacional de Investigaciones Científicas y Técnicas |
| Repositorio: | CONICET Digital (CONICET) |
| Idioma: | inglés |
| OAI Identifier: | oai:ri.conicet.gov.ar:11336/101901 |
| Acceso en línea: | http://hdl.handle.net/11336/101901 |
| Access Level: | acceso abierto |
| Palabra clave: | Lactobacillus plantarum BACTERIOPHAGE INACTIVATION HEAT TREATMENT BIOCIDES PHOTOCATALYSIS https://purl.org/becyt/ford/2.11 https://purl.org/becyt/ford/2 |
| Sumario: | The effect of several biocides, thermal treatments, and photocatalysis on the viability of four Lactobacillus plantarum phages was investigated. Times to achieve 99% inactivation (T99) of phages at 63, 72, and 90°C were evaluated in four suspension media: deMan Rogosa Sharpe broth, reconstituted skim milk, a commercial EM-glucose medium, and Tris magnesium gelatin buffer. The four phages studied were highly resistant to 63°C (T99 > 45 min); however, counts < 10 PFU/ml were achieved by heating at 90°C for 5 min. Higher thermal resistance at 72°C was observed when reconstituted skim milk and EM-glucose medium were assayed. Peracetic acid (0.15%, vol/vol) was an effective biocide for the complete inactivation of all phages studied within 5 min of exposure. Sodium hypochlorite (800 ppm) inactivated the phages completely within 30 min. Ethanol (100%) did not destroy phage particles even after 45 min. Isopropanol did not have any effect on phage viability. Phage counts < 50 PFU/ml were obtained within 180 min of photocatalytic treatment. The results obtained in this work are important for establishing adequate methods for inactivating phages in industrial plants and laboratory environments. |
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