Thermal, Chemical, and Photocatalytic Inactivation of <i>Lactobacillus plantarum</i> Bacteriophages
The effect of several biocides, thermal treatments, and photocatalysis on the viability of four <i>Lactobacillus plantarum</i> phages was investigated. Times to achieve 99% inactivation (T₉₉) of phages at 63, 72, and 90 degrees C were evaluated in four suspension media: deMan Rogosa Shar...
| Autores: | , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2009 |
| País: | Argentina |
| Institución: | Universidad Nacional de La Plata |
| Repositorio: | SEDICI (UNLP) |
| Idioma: | inglés |
| OAI Identifier: | oai:sedici.unlp.edu.ar:10915/144574 |
| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/144574 |
| Access Level: | acceso abierto |
| Palabra clave: | Biología Química Lactobacillus plantarum biocides thermal treatments photocatalysis |
| Sumario: | The effect of several biocides, thermal treatments, and photocatalysis on the viability of four <i>Lactobacillus plantarum</i> phages was investigated. Times to achieve 99% inactivation (T₉₉) of phages at 63, 72, and 90 degrees C were evaluated in four suspension media: deMan Rogosa Sharpe broth, reconstituted skim milk, a commercial EM-glucose medium, and Tris magnesium gelatin buffer. The four phages studied were highly resistant to 63 degrees C (T₉₉ > 45 min); however, counts < 10 PFU/ml were achieved by heating at 90 degrees C for 5 min. Higher thermal resistance at 72 degrees C was observed when reconstituted skim milk and EM-glucose medium were assayed. Peracetic acid (0.15%, vol/vol) was an effective biocide for the complete inactivation of all phages studied within 5 min of exposure. Sodium hypochlorite (800 ppm) inactivated the phages completely within 30 min. Ethanol (100%) did not destroy phage particles even after 45 min. Isopropanol did not have any effect on phage viability. Phage counts < 50 PFU/ml were obtained within 180 min of photocatalytic treatment. The results obtained in this work are important for establishing adequate methods for inactivating phages in industrial plants and laboratory environments. |
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