Resistance of two temperate Lactobacillus paracasei bacteriophages to high pressure homogenization, thermal treatments and chemical biocides of industrial application
Temperate bacteriophages iLp84 and iLp1308, previously isolated from mitomycin C-induction of Lactobacillus paracasei strains 84 and CNRZ1308, respectively, were tested for their resistance to several physical and chemical treatments applied in dairy industry. Long-term survival at 4ºC, -20ºC and -8...
| Autores: | , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2012 |
| País: | Argentina |
| Institución: | Consejo Nacional de Investigaciones Científicas y Técnicas |
| Repositorio: | CONICET Digital (CONICET) |
| Idioma: | inglés |
| OAI Identifier: | oai:ri.conicet.gov.ar:11336/103196 |
| Acceso en línea: | http://hdl.handle.net/11336/103196 |
| Access Level: | acceso abierto |
| Palabra clave: | LACTOBACILLUS PARACASEI BIOCIDES PHAGE SURVIVAL PHAGE INACTIVATION https://purl.org/becyt/ford/2.11 https://purl.org/becyt/ford/2 |
| Sumario: | Temperate bacteriophages iLp84 and iLp1308, previously isolated from mitomycin C-induction of Lactobacillus paracasei strains 84 and CNRZ1308, respectively, were tested for their resistance to several physical and chemical treatments applied in dairy industry. Long-term survival at 4ºC, -20ºC and -80ºC, resistance to either thermal treatments of 63ºC, 72ºC and 90ºC, high pressure homogenization (HPH, 100 MPa) or classic (ethanol, sodium hypochlorite and peracetic acid) and new commercial sanitizers, namely A (quaternary ammonium chloride), B (hydrogen peroxide, peracetic acid and peroctanoic acid), C (alkaline chloride foam), D (p-toluensulfonchloroamide, sodium salt) and E (ethoxylated nonylphenol and phosphoric acid), were determined. Phages were almost completely inactivated after eight months of storage at 25ºC, but viability was not affected at 4ºC, -20ºC or -80ºC. Both phages tolerated well HPH treatments. Phage iLp1308 showed higher thermal resistance than iLp84, but neither resisted 90ºC for 2 min. Best chemical inactivation was accomplished using peracetic acid or biocides A, C and E, whereas biocides B and D were completely ineffective. These results help to improve selection of chemical agents and physical treatments to effectively fight against phage infections in dairy plants. |
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