USE OF LOW QUALITY BARLEY STARCH FOR THE PRODUCTION OF AMYLOLYTIC ENZYMES BY Aureobasidium pullulans
Low quality barley that has been rejected for the malt production process can be used to produce value added products, particularly enzymes. Hence, in this work the starch was extracted and used to produce amylolytic enzymes by Aureobasidium pullulans. Using 50 g/L of barley raw starch, glucoamylase...
| Autores: | , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2019 |
| País: | México |
| Institución: | UNIVERSIDAD NACIONAL AUTÓNOMA DE MÉXICO |
| Repositorio: | Revista Internacional de Contaminación Ambiental |
| Idioma: | español |
| OAI Identifier: | oai:ojs.pkp.sfu.ca:article/52973 |
| Acceso en línea: | https://www.revistascca.unam.mx/rica/index.php/rica/article/view/RICA.2019.35.02.14 |
| Access Level: | acceso abierto |
| Palabra clave: | sacarificación glucoamilasa α-amilasa α-glucosidasa β-glucosidasa saccharification α-amylase glucoamylase α-glucosidase β-glucosidase |
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USE OF LOW QUALITY BARLEY STARCH FOR THE PRODUCTION OF AMYLOLYTIC ENZYMES BY Aureobasidium pullulans APROVECHAMIENTO DE ALMIDÓN DE CEBADA DE MALA CALIDAD PARA LA PRODUCCIÓN DE ENZIMAS AMILOLÍTICAS POR Aureobasidium pullulans |
| title |
USE OF LOW QUALITY BARLEY STARCH FOR THE PRODUCTION OF AMYLOLYTIC ENZYMES BY Aureobasidium pullulans |
| spellingShingle |
USE OF LOW QUALITY BARLEY STARCH FOR THE PRODUCTION OF AMYLOLYTIC ENZYMES BY Aureobasidium pullulans Hernández-Castillo, María Isabel sacarificación glucoamilasa α-amilasa α-glucosidasa β-glucosidasa saccharification α-amylase glucoamylase α-glucosidase β-glucosidase |
| title_short |
USE OF LOW QUALITY BARLEY STARCH FOR THE PRODUCTION OF AMYLOLYTIC ENZYMES BY Aureobasidium pullulans |
| title_full |
USE OF LOW QUALITY BARLEY STARCH FOR THE PRODUCTION OF AMYLOLYTIC ENZYMES BY Aureobasidium pullulans |
| title_fullStr |
USE OF LOW QUALITY BARLEY STARCH FOR THE PRODUCTION OF AMYLOLYTIC ENZYMES BY Aureobasidium pullulans |
| title_full_unstemmed |
USE OF LOW QUALITY BARLEY STARCH FOR THE PRODUCTION OF AMYLOLYTIC ENZYMES BY Aureobasidium pullulans |
| title_sort |
USE OF LOW QUALITY BARLEY STARCH FOR THE PRODUCTION OF AMYLOLYTIC ENZYMES BY Aureobasidium pullulans |
| dc.creator.none.fl_str_mv |
Hernández-Castillo, María Isabel Hernández-Navarrete, Angélica Jiménez-Montejo, Fabiola Eloisa Cruz-López, María del Carmen Hidalgo-Lara, María Eugenia López y López, Víctor Eric |
| author |
Hernández-Castillo, María Isabel |
| author_facet |
Hernández-Castillo, María Isabel Hernández-Navarrete, Angélica Jiménez-Montejo, Fabiola Eloisa Cruz-López, María del Carmen Hidalgo-Lara, María Eugenia López y López, Víctor Eric |
| author_role |
author |
| author2 |
Hernández-Navarrete, Angélica Jiménez-Montejo, Fabiola Eloisa Cruz-López, María del Carmen Hidalgo-Lara, María Eugenia López y López, Víctor Eric |
| author2_role |
author author author author author |
| dc.subject.none.fl_str_mv |
sacarificación glucoamilasa α-amilasa α-glucosidasa β-glucosidasa saccharification α-amylase glucoamylase α-glucosidase β-glucosidase |
| topic |
sacarificación glucoamilasa α-amilasa α-glucosidasa β-glucosidasa saccharification α-amylase glucoamylase α-glucosidase β-glucosidase |
| description |
Low quality barley that has been rejected for the malt production process can be used to produce value added products, particularly enzymes. Hence, in this work the starch was extracted and used to produce amylolytic enzymes by Aureobasidium pullulans. Using 50 g/L of barley raw starch, glucoamylase, α-amylase, α glucosidase and β-glucosidase enzymes were produced with maximum activities of 193, 7.4, 8.1 and 8.5 U/mL, respectively. After 30 h of fermentation a crude extract was obtained, which was used as enzymatic source for the saccharification of starch from three sources at 10 g/L: barley, soluble (from potato) and corn. First, two reaction solvents (water and acetate buffer) and the addition of calcium (Ca2+) were evaluated. Afterwards, starch gelatinization was evaluated and finally experiments varying the starch concentration (from 50 to 400 g/L) were conducted. Using water as solvent and the Ca2+ addition had a positive effect, resulting in a maximum glucose concentration of 3.84, 0.84 and 0.4 g/L from barley, soluble and corn starches, respectively. In the other hand, starch gelatinization was positive for soluble and corn starches, enhancing hydrolysis in 23 and 25 %, respectively. Increasing the starch concentration to 50 g/L was the best condition, enhancing hydrolysis in 3.2, 40 and 42 % from barley, soluble and corn starches, respectively. This work demonstrates that low quality raw barley starch can be used for the production of value added products such as enzymes. |
| publishDate |
2019 |
| dc.date.none.fl_str_mv |
2019-04-30 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion ART |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
https://www.revistascca.unam.mx/rica/index.php/rica/article/view/RICA.2019.35.02.14 10.20937/RICA.2019.35.02.14 |
| url |
https://www.revistascca.unam.mx/rica/index.php/rica/article/view/RICA.2019.35.02.14 |
| identifier_str_mv |
10.20937/RICA.2019.35.02.14 |
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spa |
| language |
spa |
| dc.relation.none.fl_str_mv |
https://www.revistascca.unam.mx/rica/index.php/rica/article/view/RICA.2019.35.02.14/46838 |
| dc.rights.none.fl_str_mv |
Derechos de autor 2019 Revista Internacional de Contaminación Ambiental https://creativecommons.org/licenses/by-nc/4.0 info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
Derechos de autor 2019 Revista Internacional de Contaminación Ambiental https://creativecommons.org/licenses/by-nc/4.0 |
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openAccess |
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application/pdf |
| dc.publisher.none.fl_str_mv |
Instituto de Ciencias de la Atmósfera, Universidad Nacional Autónoma de México |
| publisher.none.fl_str_mv |
Instituto de Ciencias de la Atmósfera, Universidad Nacional Autónoma de México |
| dc.source.none.fl_str_mv |
Revista Internacional de Contaminación Ambiental; Vol. 35 Núm. 2 (2019); 435-446 Revista Internacional de Contaminación Ambiental; Vol. 35 No. 2 (2019); 435-446 0188-4999 reponame:Revista Internacional de Contaminación Ambiental instname:UNIVERSIDAD NACIONAL AUTÓNOMA DE MÉXICO instacron:UNAM |
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UNIVERSIDAD NACIONAL AUTÓNOMA DE MÉXICO |
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UNAM |
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UNAM |
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Revista Internacional de Contaminación Ambiental |
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Revista Internacional de Contaminación Ambiental |
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1858175448130781184 |
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USE OF LOW QUALITY BARLEY STARCH FOR THE PRODUCTION OF AMYLOLYTIC ENZYMES BY Aureobasidium pullulansAPROVECHAMIENTO DE ALMIDÓN DE CEBADA DE MALA CALIDAD PARA LA PRODUCCIÓN DE ENZIMAS AMILOLÍTICAS POR Aureobasidium pullulans Hernández-Castillo, María IsabelHernández-Navarrete, AngélicaJiménez-Montejo, Fabiola EloisaCruz-López, María del CarmenHidalgo-Lara, María EugeniaLópez y López, Víctor Ericsacarificaciónglucoamilasaα-amilasaα-glucosidasaβ-glucosidasasaccharificationα-amylaseglucoamylaseα-glucosidaseβ-glucosidaseLow quality barley that has been rejected for the malt production process can be used to produce value added products, particularly enzymes. Hence, in this work the starch was extracted and used to produce amylolytic enzymes by Aureobasidium pullulans. Using 50 g/L of barley raw starch, glucoamylase, α-amylase, α glucosidase and β-glucosidase enzymes were produced with maximum activities of 193, 7.4, 8.1 and 8.5 U/mL, respectively. After 30 h of fermentation a crude extract was obtained, which was used as enzymatic source for the saccharification of starch from three sources at 10 g/L: barley, soluble (from potato) and corn. First, two reaction solvents (water and acetate buffer) and the addition of calcium (Ca2+) were evaluated. Afterwards, starch gelatinization was evaluated and finally experiments varying the starch concentration (from 50 to 400 g/L) were conducted. Using water as solvent and the Ca2+ addition had a positive effect, resulting in a maximum glucose concentration of 3.84, 0.84 and 0.4 g/L from barley, soluble and corn starches, respectively. In the other hand, starch gelatinization was positive for soluble and corn starches, enhancing hydrolysis in 23 and 25 %, respectively. Increasing the starch concentration to 50 g/L was the best condition, enhancing hydrolysis in 3.2, 40 and 42 % from barley, soluble and corn starches, respectively. This work demonstrates that low quality raw barley starch can be used for the production of value added products such as enzymes.La cebada de baja calidad rechazada para el proceso de producción de malta puede ser utilizada para la obtención de productos de valor agregado, particularmente enzimas. En este trabajo se extrajo almidón de cebada y se usó para producir enzimas amilolíticas por Aureobasidium pullulans. Utilizando 50 g/L de almidón, se produjeron glucoamilasa, α-amilasa, α-glucosidasa y β-glucosidasa con actividades máximas de 193, 7.4, 8.1 y 8.5 U/mL, respectivamente. A las 30 h de fermentación se obtuvo un extracto enzimático crudo que se utilizó para sacarificar almidón de tres fuentes a 10 g/L: cebada, soluble (papa) y maíz. Primero, fueron evaluados dos medios de reacción (agua y solución tampón de acetato) y la adición de calcio (Ca2+). Posteriormente, se evaluó la gelatinización del almidón y finalmente se realizaron experimentos aumentando la concentración de almidón (50 a 400 g/L). El agua y la adición de Ca2+ tuvieron un efecto positivo, resultando una concentración máxima de glucosa de 3.84, 0.84 y 0.4 g/L para almidón de cebada, soluble y de maíz, respectivamente. La gelatinización del almidón fue positiva para el almidón soluble y de maíz, aumentando la hidrólisis en 23 y 25 %, respectivamente. El incremento de la concentración de almidón a 50 g/L fue la mejor condición; aumentó la hidrólisis en 3.2, 40 y 42 % para almidón de cebada, soluble y de maíz, respectivamente. Este trabajo demuestra que el almidón de cebada de baja calidad puede ser aprovechado para la obtención de productos de valor agregado, como las enzimas.Instituto de Ciencias de la Atmósfera, Universidad Nacional Autónoma de México2019-04-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionARTapplication/pdfhttps://www.revistascca.unam.mx/rica/index.php/rica/article/view/RICA.2019.35.02.1410.20937/RICA.2019.35.02.14Revista Internacional de Contaminación Ambiental; Vol. 35 Núm. 2 (2019); 435-446Revista Internacional de Contaminación Ambiental; Vol. 35 No. 2 (2019); 435-4460188-4999reponame:Revista Internacional de Contaminación Ambientalinstname:UNIVERSIDAD NACIONAL AUTÓNOMA DE MÉXICOinstacron:UNAMspahttps://www.revistascca.unam.mx/rica/index.php/rica/article/view/RICA.2019.35.02.14/46838Derechos de autor 2019 Revista Internacional de Contaminación Ambientalhttps://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessoai:ojs.pkp.sfu.ca:article/529732024-08-16T19:00:37Z |
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15.812429 |