Efecto del malatión en la maduración in vitro de ovocitos porcinos y su asociación con el estrés oxidante
Malathion is one of the most commonly used insecticides, recent findings have demonstrated that it induces oxidative stress in somatic cells, but there are not enough studies demonstrating this effect in germ cells. Malathion impairs porcine oocyte viability and maturation, but studies have not show...
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| Tipo de recurso: | tesis doctoral |
| Estado: | Versión publicada |
| Fecha de publicación: | 2017 |
| País: | México |
| Institución: | Universidad Autónoma Metropolitana |
| Repositorio: | Repositorio Institucional de la UAM Iztapalapa |
| Idioma: | español |
| OAI Identifier: | oai:bindani.izt.uam.mx:js956f80d |
| Acceso en línea: | https://doi.org/10.24275/uami.js956f80d |
| Access Level: | acceso abierto |
| Palabra clave: | info:eu-repo/classification/LEM/Fertilización in vitro info:eu-repo/classification/LEM/Fertilization in vitro info:eu-repo/classification/LEM/Swine -- Reproduction info:eu-repo/classification/LEM/Cerdos -- Reproducción info:eu-repo/classification/cti/2 |
| Sumario: | Malathion is one of the most commonly used insecticides, recent findings have demonstrated that it induces oxidative stress in somatic cells, but there are not enough studies demonstrating this effect in germ cells. Malathion impairs porcine oocyte viability and maturation, but studies have not shown how oxidative stress affects maturation and which biochemical mechanisms are affected in this process in cumulus cell oocyte complexes (COCs). The aim of the present study was to determine the effect of oxidative stress produced by malathion in porcine COCs matured in vitro. Different concentrations of malathion: 0, 750 µM (IMC50) and sub-lethal of 1000 µM, they were used to evaluate the content of antioxidant enzymes, reactive oxygen species (ROS), protein oxidation, and lipid peroxidation (LPO). COCs viability and oocyte maturation decreased in a concentrationdependent manner. Malathion increased superoxide dismutase (SOD), glutathione-Stransferase (GST) and glucose 6 phosphate dehydrogenase (G6PD) content, and decreased glutathione peroxidase (GSH-Px) and catalase (CAT) content. ROS, protein oxidation and TBARS levels increased in COCs exposed to the insecticide, but when COCs were pretreated with the antioxidant trolox (50 µM) before malathion exposure, these parameters decreased. This study showed that malathion has a detrimental effect on COCs during in vitro maturation (IVM), inducing oxidative stress, while trolox prevented malathion toxicity by decreasing oxidative damage. |
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