Efecto del metil-parabeno durante la maduración in vitro de ovocitos de cerdo
Background: Parabens (PBs) are compounds widely used in food and personal care products industry as antimicrobials and preservatives. Their indiscriminate use has allowed them to be detected in different ecosystems so that humans and other organisms are highly exposed. Methylparaben (MePB), compared...
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| Tipo de recurso: | tesis de maestría |
| Estado: | Versión publicada |
| Fecha de publicación: | 2020 |
| País: | México |
| Institución: | Universidad Autónoma Metropolitana |
| Repositorio: | Repositorio Institucional de la UAM Iztapalapa |
| Idioma: | español |
| OAI Identifier: | oai:bindani.izt.uam.mx:wd375w449 |
| Acceso en línea: | https://doi.org/10.24275/uami.wd375w449 |
| Access Level: | acceso abierto |
| Palabra clave: | info:eu-repo/classification/LEM/Reproducción animal info:eu-repo/classification/LEM/Cerdos info:eu-repo/classification/LEM/Fertilization in vitro info:eu-repo/classification/LEM/Fertilización in vitro info:eu-repo/classification/LEM/Swine -- Reproduction info:eu-repo/classification/LEM/Reproduction info:eu-repo/classification/LEM/Metil Parabeno info:eu-repo/classification/cti/6 |
| Sumario: | Background: Parabens (PBs) are compounds widely used in food and personal care products industry as antimicrobials and preservatives. Their indiscriminate use has allowed them to be detected in different ecosystems so that humans and other organisms are highly exposed. Methylparaben (MePB), compared with other PBs, is mostly detected in food, personal, and baby care products. PBs could be linked to hormonal disorders generation and fertility impairment since they have been recently classified as endocrine disruptors. The knowledge of the effects that MePB can exert is of great importance since, in terms of reproduction, information is limited. Therefore, the objective of this study was to evaluate the effect of MePB on concentrations of 0, 50, 100, 500, 750 y 1000 μM porcine oocyte viability and in vitromaturation(IVM), as well as to determine the lethal concentration 50 (LC50) and maturation inhibition concentration 50 (MIC50). Results:AfterIVM, dispersed cumulus cells and cytoplasmic alterations were observed in oocytes exposed to MePB; however, viability was not affected in the concentration range assayed. In addition, oocyte maturation decreased in a concentration-dependent manner after exposure to MePB. The estimated LC50was 2028.38 μM, whereas MIC50was 780.31 μM. Conclusions: As far as we know, this is the first study demonstrating that MePB can cause deleterious effects to the in vitro maturation of porcine oocytes. This study demonstratesthat MePB altered porcine oocytes morphology and caused cumulus cells dispersion, which resulted in decreased oocyte maturation. Therefore, MePB exposure may be one of the factors involved in fertility impairment in mammals, including the human. |
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