Self-association of enolase from Trichomonas vaginalis. monomers, dimers, and octamers coexist in solution
"We used small-angle X-ray scattering to study the self-association of enolase from Trichomonas vaginalis as a function of the protein concentration and cosolute type. We observed coexisting monomers, dimers, and octamers in variable relative populations, depending on whether Tris–acetate, Tris...
| Autores: | , , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2018 |
| País: | México |
| Institución: | Instituto Potosino de Investigación Científica y Tecnológica |
| Repositorio: | Repositorio Institucional del IPICYT |
| OAI Identifier: | oai:ipicyt.repositorioinstitucional.mx:1010/1919 |
| Acceso en línea: | http://ipicyt.repositorioinstitucional.mx/jspui/handle/1010/1919 |
| Access Level: | acceso abierto |
| Palabra clave: | info:eu-repo/classification/Autor/Plasminogen-binding info:eu-repo/classification/Autor/Yeast enolase info:eu-repo/classification/Autor/Triosephosphate isomerase info:eu-repo/classification/Autor/Streptococcus-pneumoniae info:eu-repo/classification/Autor/Quaternary structure info:eu-repo/classification/Autor/Mammalian enolases info:eu-repo/classification/Autor/Alpha enolase info:eu-repo/classification/Autor/Protein info:eu-repo/classification/Autor/Dissociation info:eu-repo/classification/Autor/Scattering info:eu-repo/classification/cti/2 info:eu-repo/classification/cti/23 |
| Sumario: | "We used small-angle X-ray scattering to study the self-association of enolase from Trichomonas vaginalis as a function of the protein concentration and cosolute type. We observed coexisting monomers, dimers, and octamers in variable relative populations, depending on whether Tris–acetate, Tris–HCl, or potassium phosphate buffers were used. Phosphate ions hindered the formation of dimers and octamers. In contrast, the populations of dimers and octamers increased in Tris–acetate or Tris–HCl buffers and additionally increased by augmenting protein concentration or adding magnesium. Single oligomeric species could not be isolated in any of the experimental conditions tested. Furthermore, the secondary and tertiary structures, as well as the temperature-induced denaturation of the mixtures of species, were investigated. The acquired species lost enzymatic activity, but they were prone to interact with plasminogen, as judged from changes in the secondary and tertiary structures upon complex formation." |
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