Evaluación de la expresión de un gen de función trofoblástica (Interferón tau) en embriones producidos in vitro de ovino doméstico y de híbridos de borrego Cimarrón (Ovis canadensis mexicana)
The major signal for maternal recognition of pregnancy in the ruminant species is interferon tau (IFNT). In the present study, in vivo and in vitro domestic and hybrid blastocysts were obtained, and IFNT expression was measured by semi quantitative RT-PCR and quantitative RT-PCR method. Hybrid IVF p...
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| Tipo de recurso: | tesis doctoral |
| Estado: | Versión publicada |
| Fecha de publicación: | 2012 |
| País: | México |
| Institución: | Universidad Autónoma Metropolitana |
| Repositorio: | Repositorio Institucional de la UAM Iztapalapa |
| Idioma: | español |
| OAI Identifier: | oai:bindani.izt.uam.mx:g445cd385 |
| Acceso en línea: | https://doi.org/10.24275/uami.g445cd385 |
| Access Level: | acceso abierto |
| Palabra clave: | info:eu-repo/classification/LEM/Sheep farming info:eu-repo/classification/LEM/Fertilization in vitro info:eu-repo/classification/LEM/Bighorn sheep info:eu-repo/classification/LEM/Biología experimental info:eu-repo/classification/LEM/Ganadería ovina info:eu-repo/classification/LEM/Fertilización in vitro info:eu-repo/classification/LEM/Borrego cimarrón info:eu-repo/classification/LEM/Biology, Experimental info:eu-repo/classification/cti/2 |
| Sumario: | The major signal for maternal recognition of pregnancy in the ruminant species is interferon tau (IFNT). In the present study, in vivo and in vitro domestic and hybrid blastocysts were obtained, and IFNT expression was measured by semi quantitative RT-PCR and quantitative RT-PCR method. Hybrid IVF produced less blastocyst than domestic sheep IVF. In vivo and in vitro hybrid embryos showed lower expression of IFNT when compared with domestic blastocyst controls. Additionally, IFNT genes isolated from genomic DNA obtained from both, parental species and from a hybrid sheep were cloned and sequenced. Two novel sequences of IFNT of Bighorn sheep, two sequences of Pelibuey sheep and one sequence of a hybrid were obtained. The hybrid sequence showed a 100% of identity with one of Bighorn sheep reported in this study, concluding that, this sequence is the allele inherits from the Bighorn sheep. The sequences of Bighorn sheep and Pelibuey sheep showed high identity, 98% and 95% respectively, highest than the sequences in Ovis group reported previously. This high identity could explain the maternal recognition of pregnancy success in hybrization between these species. This study reports, for the first time, differences in IFNT expression in hybrid blastocysts and novel alleles of the IFNT gene in Bighorn sheep, Pelibuey sheep and hybrid, and demonstrates that hybrid sheep model may be a valuable tool for the study of mechanisms in maternal recognition of pregnancy in wild and domestic ungulates. |
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