Obtención de embriones de ovino por transferencia nuclear a partir de Fibroblastos de piel y ovocitos maduros in vitro con resveratrol
The aim of the present study was to evaluate the effects of different concentrations of resveratrol on ovine (Ovisaries) oocyte in vitro maturation (IVM) and subsequent embryonic development after handmade cloning (HMC). To determine the effects of resveratrol were evaluated four different concentra...
| Autor: | |
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| Tipo de recurso: | tesis de maestría |
| Estado: | Versión publicada |
| Fecha de publicación: | 2016 |
| País: | México |
| Institución: | Universidad Autónoma Metropolitana |
| Repositorio: | Repositorio Institucional de la UAM Iztapalapa |
| Idioma: | español |
| OAI Identifier: | oai:bindani.izt.uam.mx:kd17cs845 |
| Acceso en línea: | https://doi.org/10.24275/uami.kd17cs845 |
| Access Level: | acceso abierto |
| Palabra clave: | info:eu-repo/classification/LEM/Sheep -- Reproduction info:eu-repo/classification/LEM/Biotecnología animal info:eu-repo/classification/LEM/Oveja -- Reproducción info:eu-repo/classification/LEM/Animal biotechnology info:eu-repo/classification/cti/2 |
| Sumario: | The aim of the present study was to evaluate the effects of different concentrations of resveratrol on ovine (Ovisaries) oocyte in vitro maturation (IVM) and subsequent embryonic development after handmade cloning (HMC). To determine the effects of resveratrol were evaluated four different concentrations (0, 0 .5, 2 and 5 M) on the nuclear maturation, reactive oxygen species (ROS) levels, as well as early embryonic development and morphological quality. After IVM, no significant difference was observed in maturation of the 0.5 and 2 M resveratrol groups (81.3 and 72.0%, respectively) compared with the control (0 M) (74.2%), but the 5 M resveratrol group showed significantly decreased nuclear maturation (56.0%) (P<0.05). Intracellular ROS levels in oocyte matured did not show significant differences between the groups (P>0.05). In subsequent embryonic development after HMC, no significant differences were observed in cleave d embryos between the groups (81.2, 84.4, 80.1 and 77.2%, respectively) (P>0.05). In 8 - 16 cell embryos, no significant differences were observed between the groups (65.6, 70.2, 64.3 and 63.6%, respectively) (P>0.05). In morulae, the 5 M group was significantly lower (6.8%) compared with 0, 0.5 and 2 M groups (32.8, 35.1 and 28.7%, respectively) (P<0.05). But, the 0.5 M resveratrol group had significantly higher morula compactation rates (10.7%) compared with 0, 2 and 5 M groups (6.2, 0 and 0%, respectively) (P<0.05). Morphological quality did not show significant differences in 8 - 16 cell embryos between the 1, 2, 3 and 4 quality (P>0.0 5). In morulae treated with 0.5 M had significantly less quality 4 (bad) embryos (4.7%) compared with 0, 2 and 5 M (23.8, 23.3 and 33.3%, respectively) (P<0.05), but with 5 M had significantly less quality 1 (excellent) embryos (0%) compare d with 0, 0.5 and 2 M (23.8, 30.1 and 23.3%, respectively) (P<0.05). In conclusion, resvetratrol supplementation on ovine oocyte IVM did not show significant differences in maturation rates and ROS levels. However, a treatment with 0.5 M during IVM, promoted the compactation of handmade cloned morulae and improved its quality. But with 5 M had significantly less IVM rates as well as embryonic development and quality. Keywords: Handmade cloning, Resveratrol, Ovine, In vitro maturation, Embryonic development. |
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