EXPRESION DE RECEPTORES COLINÉRGICOS EXTRANEURONALES Y EVALUACION DE MECANISMOS DE INMUNIDAD INNATA CELULAR EN TILAPIA ( Oreochromis niloticus) EXPUESTA DE FORMA AGUDA A DIAZINÓN
Organophosphorus pesticides (OPs) are broad-spectrum insecticides that exert their neurotoxic effect by inhibiting the enzyme acetylcholinesterase (AChE). However, this enzyme is not only present in the nervous system; cholinergic activity has also been demonstrated in leukocytes, so OPs can modify...
| Autores: | , |
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| Tipo de recurso: | tesis doctoral |
| Estado: | Versión publicada |
| Fecha de publicación: | 2019 |
| País: | México |
| Institución: | Universidad Autónoma de Nayarit |
| Repositorio: | Repositorio Institucional Aramara de la UAN |
| Idioma: | español |
| OAI Identifier: | oai:dspace.uan.mx:123456789/2280 |
| Acceso en línea: | http://dspace.uan.mx:8080/jspui/handle/123456789/2280 |
| Access Level: | acceso abierto |
| Palabra clave: | Receptores, Colinérgicos, Inmunidad, Tilapia CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA [6] |
| Sumario: | Organophosphorus pesticides (OPs) are broad-spectrum insecticides that exert their neurotoxic effect by inhibiting the enzyme acetylcholinesterase (AChE). However, this enzyme is not only present in the nervous system; cholinergic activity has also been demonstrated in leukocytes, so OPs can modify neuroimmunological communication. The objective of this study was to determine the cholinergic alteration and parameters of innate immunity, caused by diazinon (DZN) in cells of the immune system, using the tilapia fish (Oreochromis niloticus) as a model. For this purpose, the fish were exposed in vivo for 6 or 24 h at 0.97, 1.95 and 3.91 mg/L at DZN. Immediately, the expression of acetylcholine muscarinic receptors (mAChR) (M2-M5 subtypes) was evaluated through leukocytes, through RT-qPCR, as well as parameters of the innate response, such as phagocytic capacity, production of reactive oxygen species (ROS) and formation of extracellular neutrophil traps (NETs), using fluorescent techniques, such as flow cytometry and microscopy. The results obtained indicate that DZN induces a decrease in phagocytic capacity and increased ROS production and NETs formation; alterations that could be related to a cholinergic disruption, since the decrease in the expression of all subtypes of mAChR evaluated (M2-M5) were observed. The results obtained can help the understanding of important biological phenomena, because the leukocyte cholinergic system is essential in basic physiological processes, as immune response induction, as well as behavioral-cognitive aspects, so that its alteration puts survival at risk of organism. |
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