EVALUACION DEL POTENCIAL DE MEMBRANA MITOCONDRIAL EN LINFOCITOS DE TILAPIA NILOTICA (OREOCHROMIS NILOTICUS) EXPUESTA A UN PLAGUICIDA ANTI-COLIENSTARASICO
Cholinergic components have been characterized in mammalian lymphocytes, which could play an important role in the regulation of the immune response. Organophosphate pesticides, being inhibitors of the enzyme acetylcholinesterase, can exert an immunotoxic effect through the alteration of this extra-...
| Autores: | , , , , , , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2015 |
| País: | México |
| Institución: | Universidad Autónoma de Nayarit |
| Repositorio: | Repositorio Institucional Aramara de la UAN |
| Idioma: | español |
| OAI Identifier: | oai:dspace.uan.mx:123456789/468 |
| Acceso en línea: | http://dspace.uan.mx:8080/jspui/handle/123456789/468 |
| Access Level: | acceso abierto |
| Palabra clave: | Evaluación Membrana mitocondrial Linfocitos Tilapia nilótica Plaguicida Anti-colienstarásico Evaluation Mitochondrial membrane Lymphocytes Nilotic tilapia Pesticide CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA [6] |
| Sumario: | Cholinergic components have been characterized in mammalian lymphocytes, which could play an important role in the regulation of the immune response. Organophosphate pesticides, being inhibitors of the enzyme acetylcholinesterase, can exert an immunotoxic effect through the alteration of this extra-neuronal cholinergic lymphocyte system, thus altering the physiology of the lymphocytes. In the present work the tilapia nilótica fish (Oreochromis niloticus) was used as a model organism to evaluate the effect of diazinón (organophosphate pesticide) on the mitochondrial membrane potential in lymphocytes. The fish were exposed to sublethal concentrations of diazinon (3.97, 1.95 and 0.97 ppm) for a period of 6 h, later the splenic tissue was extracted and disintegrated. Mononuclear cells were separated by density gradient, DiOC2 (20 nM) was added for 15 min at 28°C and samples were analyzed by flow cytometry. Preliminary results showed that diazinon at concentrations of 0.97, 1.95 and 3.97 ppm causes loss of membrane potential of 19, 30 and 23 % respectively in comparison to the control group. |
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