Effects of the expression of ExpReb and GFP-Kkv [Dataset]

All images are projections of confocal sections. (A, B) Overexpressed full-length Reb localises mainly apically in trachea (A) and in salivary glands (B). (C) The simultaneous overexpression of MH2-exp and GFP-kkv does not rescue the absence of extracellular chitin deposition, and it produces intrac...

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Detalles Bibliográficos
Autores: Giorgio, Ettore de, Giannios, Panagiotis, Espinàs, Maria Lluïsa, Llimargas, Marta
Tipo de recurso: conjunto de datos
Estado:Versión publicada
Fecha de publicación:2023
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/337757
Acceso en línea:http://hdl.handle.net/10261/337757
Access Level:acceso abierto
Palabra clave:Highly abundant polymer
Div >< p
Chitin structural diversity
Chitin accumulates intracellularly
kkv subcellular localisation
Fully elucidated yet
Ectodermal tissues requires
Apical extracellular matrices
Elucidated yet
Extracellular space
Apical domain
Chitin synthase
Chitin deposition
Chitin biosynthesis
Topological distribution
Rebuf reveals
Proteins expansión
Principal component
Previously showed
Multiple applications
Molecular mechanisms
Molecular análisis
Mh2 domain
Less punctae
kkv complexes
Excellent biomaterial
Dynamic interplay
Drosophila </
Conserved proteins
Conserved nα
Concomitant activities
Although kkv
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oai_identifier_str oai:digital.csic.es:10261/337757
network_acronym_str ES
network_name_str España
repository_id_str
spelling Effects of the expression of ExpReb and GFP-Kkv [Dataset]Giorgio, Ettore deGiannios, PanagiotisEspinàs, Maria LluïsaLlimargas, MartaHighly abundant polymerDiv >< pChitin structural diversityChitin accumulates intracellularlykkv subcellular localisationFully elucidated yetEctodermal tissues requiresApical extracellular matricesElucidated yetExtracellular spaceApical domainChitin synthaseChitin depositionChitin biosynthesisTopological distributionRebuf revealsProteins expansiónPrincipal componentPreviously showedMultiple applicationsMolecular mechanismsMolecular análisisMh2 domainLess punctaekkv complexesExcellent biomaterialDynamic interplayDrosophila </Conserved proteinsConserved nαConcomitant activitiesAlthough kkvAll images are projections of confocal sections. (A, B) Overexpressed full-length Reb localises mainly apically in trachea (A) and in salivary glands (B). (C) The simultaneous overexpression of MH2-exp and GFP-kkv does not rescue the absence of extracellular chitin deposition, and it produces intracellular chitin vesicles (pink arrowheads). (D) Endogenous Exp localises mainly apically in trachea, although a bit of the protein can be detected intracellularly. (E) Example of a salivary gland used to quantify the number of GFP-Kkv vesicles in Fig 5I. (E’) A mask to count the vesicles is generated by substracting background and the apical membrane region. Scale bars: 10 μm.Peer reviewedFigshareConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202320232023info:eu-repo/semantics/datasethttp://purl.org/coar/resource_type/c_ddb1Publisher's versioninfo:eu-repo/semantics/publishedVersionimage/tiffhttp://hdl.handle.net/10261/337757reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésGiorgio, Ettore de; Giannios, Panagiotis; Espinàs, Maria Lluïsa; Llimargas, Marta. A dynamic interplay between chitin synthase and the proteins Expansion/Rebuf reveals that chitin polymerisation and translocation are uncoupled in Drosophila. http://dx.doi.org/10.1371/journal.pbio.3001978 . http://hdl.handle.net/10261/334810https://doi.org/10.1371/journal.pbio.3001978.s002Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3377572026-05-22T06:33:51Z
dc.title.none.fl_str_mv Effects of the expression of ExpReb and GFP-Kkv [Dataset]
title Effects of the expression of ExpReb and GFP-Kkv [Dataset]
spellingShingle Effects of the expression of ExpReb and GFP-Kkv [Dataset]
Giorgio, Ettore de
Highly abundant polymer
Div >< p
Chitin structural diversity
Chitin accumulates intracellularly
kkv subcellular localisation
Fully elucidated yet
Ectodermal tissues requires
Apical extracellular matrices
Elucidated yet
Extracellular space
Apical domain
Chitin synthase
Chitin deposition
Chitin biosynthesis
Topological distribution
Rebuf reveals
Proteins expansión
Principal component
Previously showed
Multiple applications
Molecular mechanisms
Molecular análisis
Mh2 domain
Less punctae
kkv complexes
Excellent biomaterial
Dynamic interplay
Drosophila </
Conserved proteins
Conserved nα
Concomitant activities
Although kkv
title_short Effects of the expression of ExpReb and GFP-Kkv [Dataset]
title_full Effects of the expression of ExpReb and GFP-Kkv [Dataset]
title_fullStr Effects of the expression of ExpReb and GFP-Kkv [Dataset]
title_full_unstemmed Effects of the expression of ExpReb and GFP-Kkv [Dataset]
title_sort Effects of the expression of ExpReb and GFP-Kkv [Dataset]
dc.creator.none.fl_str_mv Giorgio, Ettore de
Giannios, Panagiotis
Espinàs, Maria Lluïsa
Llimargas, Marta
author Giorgio, Ettore de
author_facet Giorgio, Ettore de
Giannios, Panagiotis
Espinàs, Maria Lluïsa
Llimargas, Marta
author_role author
author2 Giannios, Panagiotis
Espinàs, Maria Lluïsa
Llimargas, Marta
author2_role author
author
author
dc.contributor.none.fl_str_mv Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Highly abundant polymer
Div >< p
Chitin structural diversity
Chitin accumulates intracellularly
kkv subcellular localisation
Fully elucidated yet
Ectodermal tissues requires
Apical extracellular matrices
Elucidated yet
Extracellular space
Apical domain
Chitin synthase
Chitin deposition
Chitin biosynthesis
Topological distribution
Rebuf reveals
Proteins expansión
Principal component
Previously showed
Multiple applications
Molecular mechanisms
Molecular análisis
Mh2 domain
Less punctae
kkv complexes
Excellent biomaterial
Dynamic interplay
Drosophila </
Conserved proteins
Conserved nα
Concomitant activities
Although kkv
topic Highly abundant polymer
Div >< p
Chitin structural diversity
Chitin accumulates intracellularly
kkv subcellular localisation
Fully elucidated yet
Ectodermal tissues requires
Apical extracellular matrices
Elucidated yet
Extracellular space
Apical domain
Chitin synthase
Chitin deposition
Chitin biosynthesis
Topological distribution
Rebuf reveals
Proteins expansión
Principal component
Previously showed
Multiple applications
Molecular mechanisms
Molecular análisis
Mh2 domain
Less punctae
kkv complexes
Excellent biomaterial
Dynamic interplay
Drosophila </
Conserved proteins
Conserved nα
Concomitant activities
Although kkv
description All images are projections of confocal sections. (A, B) Overexpressed full-length Reb localises mainly apically in trachea (A) and in salivary glands (B). (C) The simultaneous overexpression of MH2-exp and GFP-kkv does not rescue the absence of extracellular chitin deposition, and it produces intracellular chitin vesicles (pink arrowheads). (D) Endogenous Exp localises mainly apically in trachea, although a bit of the protein can be detected intracellularly. (E) Example of a salivary gland used to quantify the number of GFP-Kkv vesicles in Fig 5I. (E’) A mask to count the vesicles is generated by substracting background and the apical membrane region. Scale bars: 10 μm.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/dataset
http://purl.org/coar/resource_type/c_ddb1
Publisher's version
info:eu-repo/semantics/publishedVersion
format dataset
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/337757
url http://hdl.handle.net/10261/337757
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Giorgio, Ettore de; Giannios, Panagiotis; Espinàs, Maria Lluïsa; Llimargas, Marta. A dynamic interplay between chitin synthase and the proteins Expansion/Rebuf reveals that chitin polymerisation and translocation are uncoupled in Drosophila. http://dx.doi.org/10.1371/journal.pbio.3001978 . http://hdl.handle.net/10261/334810
https://doi.org/10.1371/journal.pbio.3001978.s002
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv image/tiff
dc.publisher.none.fl_str_mv Figshare
publisher.none.fl_str_mv Figshare
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869425720534499328
score 15,812429

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