Analysis of the role of the CM2 domain of Exp/Reb [Dataset]
(A) Alignment of amino acids (aa) sequences of the isoform B of Exp (aa 356–433) and homologs; the blue square indicates 8 aa highly conserved, the red square includes 9 aa less conserved. (B-D) Show projections of confocal sections and (E-F) show single confocal sections. (B) The overexpression of...
| Autores: | , , , |
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| Tipo de recurso: | conjunto de datos |
| Estado: | Versión publicada |
| Fecha de publicación: | 2023 |
| País: | España |
| Institución: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/337701 |
| Acceso en línea: | http://hdl.handle.net/10261/337701 |
| Access Level: | acceso abierto |
| Palabra clave: | Highly abundant polymer Div >< p Chitin structural diversity Chitin accumulates intracellularly kkv subcellular localisation Fully elucidated yet Ectodermal tissues requires Apical extracellular matrices Elucidated yet Extracellular space Apical domain Chitin synthase Chitin deposition Chitin biosynthesis Topological distribution Rebuf reveals Proteins expansión Principal component Previously showed Multiple applications Molecular mechanisms Molecular análisis Mh2 domain Less punctae kkv complexes Excellent biomaterial Dynamic interplay Drosophila </ Conserved proteins Conserved nα Concomitant activities Although kkv |
| Sumario: | (A) Alignment of amino acids (aa) sequences of the isoform B of Exp (aa 356–433) and homologs; the blue square indicates 8 aa highly conserved, the red square includes 9 aa less conserved. (B-D) Show projections of confocal sections and (E-F) show single confocal sections. (B) The overexpression of expΔCM2 in an exp reb mutant background rescues the lack of extracellular chitin deposition. (C, D) The simultaneous expression of expΔCM2 and GFP-kkv produces morphogenetic defects in the trachea (arrowheads in C) and ectopic chitin deposition in the lumen of salivary glands (D). (E, F) Overexpressed Exp localises mainly in the apical region (orange arrowheads) with respect the basal domain (yellow arrowheads), while the apical accumulation of overexpressed ExpΔCM2 is less conspicuous (F). (G) Quantifications of accumulation of Exp and ExpΔCM2 in apical versus basal region. n corresponds to the number of ratios analysed (apical/basal ratio per cell), and brackets indicate the number of embryos used. Ratios were obtained from the apical (orange line in E) and basal (yellow line in E) domains of single cells in trachea and salivary glands. The underlying data for quantifications can be found in the S1 Data. Scale bars B, C: 25 μm; D-F: 10 μm. |
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