Effects of the expression of ExpReb and GFP-Kkv [Dataset]
All images are projections of confocal sections. (A, B) Overexpressed full-length Reb localises mainly apically in trachea (A) and in salivary glands (B). (C) The simultaneous overexpression of MH2-exp and GFP-kkv does not rescue the absence of extracellular chitin deposition, and it produces intrac...
| Autores: | , , , |
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| Tipo de recurso: | conjunto de datos |
| Estado: | Versión publicada |
| Fecha de publicación: | 2023 |
| País: | España |
| Institución: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/337757 |
| Acceso en línea: | http://hdl.handle.net/10261/337757 |
| Access Level: | acceso abierto |
| Palabra clave: | Highly abundant polymer Div >< p Chitin structural diversity Chitin accumulates intracellularly kkv subcellular localisation Fully elucidated yet Ectodermal tissues requires Apical extracellular matrices Elucidated yet Extracellular space Apical domain Chitin synthase Chitin deposition Chitin biosynthesis Topological distribution Rebuf reveals Proteins expansión Principal component Previously showed Multiple applications Molecular mechanisms Molecular análisis Mh2 domain Less punctae kkv complexes Excellent biomaterial Dynamic interplay Drosophila </ Conserved proteins Conserved nα Concomitant activities Although kkv |
| Sumario: | All images are projections of confocal sections. (A, B) Overexpressed full-length Reb localises mainly apically in trachea (A) and in salivary glands (B). (C) The simultaneous overexpression of MH2-exp and GFP-kkv does not rescue the absence of extracellular chitin deposition, and it produces intracellular chitin vesicles (pink arrowheads). (D) Endogenous Exp localises mainly apically in trachea, although a bit of the protein can be detected intracellularly. (E) Example of a salivary gland used to quantify the number of GFP-Kkv vesicles in Fig 5I. (E’) A mask to count the vesicles is generated by substracting background and the apical membrane region. Scale bars: 10 μm. |
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