Rice seeds as biofactories of the production of antimicrobial peptides
A series of antimicrobial peptides (AMPs) derived from the BP134 peptide (a member of the CECMEL11 library) exhibited potent bactericidal effect and low cytotoxicity, and were selected for expression in transgenic rice. Transgenic plants were generated expressing a codon-optimized AMP, under the con...
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| Tipo de recurso: | tesis doctoral |
| Estado: | Versión publicada |
| Fecha de publicación: | 2014 |
| País: | España |
| Institución: | CBUC, CESCA |
| Repositorio: | TDR. Tesis Doctorales en Red |
| OAI Identifier: | oai:www.tdx.cat:10803/135054 |
| Acceso en línea: | http://hdl.handle.net/10803/135054 |
| Access Level: | acceso abierto |
| Palabra clave: | CECMELL11 peptides Biopesticides Biopesticidas Heterologous expression Expressió heteròloga Expresión heteróloga Rice endosperm Endosperma d'arròs Endosperma de arroz Rice embryo Embrió d'arròs Embión de arroz Pathogen resistance Resistència a patògens Resistencia a patógenos Antimicrobial peptides Pèptids antimicrobians Péptidos antimicrobianos 577 632 |
| Sumario: | A series of antimicrobial peptides (AMPs) derived from the BP134 peptide (a member of the CECMEL11 library) exhibited potent bactericidal effect and low cytotoxicity, and were selected for expression in transgenic rice. Transgenic plants were generated expressing a codon-optimized AMP, under the control of a seed-specific promoter. It was confirmed that the expected peptides accumulated in the transgenic rice seeds, but not in other plant tissues, resulting in enhanced levels of protection against bacterial and fungal plant pathogens. Procedures for purification of the AMPs from seeds have been established based on solubilization/selective precipitation and Weak Cation Exchange-Reverse Phase chromatography. One of the peptides obtained, the S-Cec A, exhibited a high antibacterial and bactericidal activity against Dickeya sp. The accumulation levels of the recombinant AMP peptides in rice seeds were dependent on the strategy used, and its production in seeds offers a promising perspective for the use of rice as biofactory |
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