Evaluación de los efectos no intencionados de los transgenes en plantas modificadas genéticamente (MG) resistentes a plagas y diseñadas como biofactorías de péptidos antimicrobianos
Genetically modified crops are submitted to strict regulation to ensure the safety of consumers and the environment. To complement the comparison between GM plants and their counterparts, in the present Thesis, we evaluated the possible unexpected effects of the transgene on the host plant, by means...
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| Tipo de recurso: | tesis doctoral |
| Estado: | Versión publicada |
| Fecha de publicación: | 2012 |
| País: | España |
| Institución: | CBUC, CESCA |
| Repositorio: | TDR. Tesis Doctorales en Red |
| OAI Identifier: | oai:www.tdx.cat:10803/97166 |
| Acceso en línea: | http://hdl.handle.net/10803/97166 |
| Access Level: | acceso abierto |
| Palabra clave: | Organismes modificats genèticament Organismos modificados genéticamente Genetically modified organisms Pèptids antimicrobians Péptidos antimicrobianos Antimicrobial peptides Efectes no intencionats del transgèn Efectos no intencionados del transgén Unexpected effects of the transgene Análisis generalista Profiling Anàlisi generalista Arrós modificat genèticament resistent a patògens Arroz modificado genéticamente resistente a patógenos Genetically modified rice pathogen resistant Expressió heterologa en planta Expressión heteróloga en planta Heterologous expression in plant 575 633 |
| Sumario: | Genetically modified crops are submitted to strict regulation to ensure the safety of consumers and the environment. To complement the comparison between GM plants and their counterparts, in the present Thesis, we evaluated the possible unexpected effects of the transgene on the host plant, by means of transcriptomic technologies. More exactly, we studied three pathogen-resistant GM rice lines: S-afp, expressing constitutively the antifungal protein AFP; and S-bp217 and S-bp213, expressing undecapeptide BP100 derivatives, which were developed in the UdG in the context of this Thesis. Although the high phytotoxicity of the BP100 derivatives on the host plant the transcriptional changes observed in S-afp, S-bp217 and S-bp213 compared to the conventional line Senia were similar that those observed in other GM crops, of other species and with different transgenes, and only the half of them was attributed to the insertion and/or expression of the transgene. |
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