Unambiguous detection of atherosclerosis using bioorthogonal nanomaterials

The importance of atherosclerosis is driving research to create improved diagnostic tools based on molecular imaging. Pretargeted imaging is the use of bioorthogonal probes that selectively accumulate upon reaction with a pre-modified biomolecule in vivo. To date, this very promising approach has no...

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Autores: Pellico, Juan, Fernández-Barahona, Irene, Benito, Marina, Gaitán-Simón, Ángel, Gutiérrez, Lucía, Ruiz-Cabello, Jesús, Herranz, Fernando
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2019
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/176957
Acceso en línea:http://hdl.handle.net/10261/176957
Access Level:acceso abierto
Palabra clave:Molecular imaging
Iron oxide
Gallium-68
Atherosclerosis
Nano-radiomaterials
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spelling Unambiguous detection of atherosclerosis using bioorthogonal nanomaterialsPellico, JuanFernández-Barahona, IreneBenito, MarinaGaitán-Simón, ÁngelGutiérrez, LucíaRuiz-Cabello, JesúsHerranz, FernandoMolecular imagingIron oxideGallium-68AtherosclerosisNano-radiomaterialsThe importance of atherosclerosis is driving research to create improved diagnostic tools based on molecular imaging. Pretargeted imaging is the use of bioorthogonal probes that selectively accumulate upon reaction with a pre-modified biomolecule in vivo. To date, this very promising approach has not been applied to atherosclerosis. Neither has been the use of a single nano-radiomaterial for PET / T-MR imaging of atherosclerosis. Here, we synthesized bioorthogonal nano-radiomaterials for in vivo pretargeted molecular imaging in a mouse model of atherosclerosis. Based on tetrazine-ligation, these functionalized Ga iron oxide nano-radiomaterials provide simultaneous PET and T-MRI signals and selectively accumulate in atherosclerotic plaques in mice sequentially injected with trans-cyclooctene-modified antibodies against oxidized LDL followed by the hybrid nano-radiomaterial. Our results demonstrate the ability of this approach to unambiguously detect atherosclerosis. Furthermore, we show the first example of how hybrid imaging can be used for pretargeted bioorthogonal molecular imaging with nanomaterials.This work was funded by the Spanish Ministry for Economy and Competitiveness (MEyC) (grant number: SAF2016-79593-P and SAF2017-84494-C2-R) and Carlos III Health Institute (grant number: DTS16/00059). L.G. was funded by Ramon y Cajal grant (RYC-2014-15512).Peer ReviewedElsevierAgencia Estatal de Investigación (España)Ministerio de Economía y Competitividad (España)Instituto de Salud Carlos IIIMinisterio de Ciencia, Innovación y Universidades (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2019201920192019info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/176957reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#SAF2017-84494-C2-R/AEI/10.13039/501100011033info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/SAF2016-79593-Pinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/SAF2017-84494-C2-Rinfo:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/RYC-2014-15512http://dx.doi.org/10.1016/j.nano.2018.12.015Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1769572026-05-22T06:33:51Z
dc.title.none.fl_str_mv Unambiguous detection of atherosclerosis using bioorthogonal nanomaterials
title Unambiguous detection of atherosclerosis using bioorthogonal nanomaterials
spellingShingle Unambiguous detection of atherosclerosis using bioorthogonal nanomaterials
Pellico, Juan
Molecular imaging
Iron oxide
Gallium-68
Atherosclerosis
Nano-radiomaterials
title_short Unambiguous detection of atherosclerosis using bioorthogonal nanomaterials
title_full Unambiguous detection of atherosclerosis using bioorthogonal nanomaterials
title_fullStr Unambiguous detection of atherosclerosis using bioorthogonal nanomaterials
title_full_unstemmed Unambiguous detection of atherosclerosis using bioorthogonal nanomaterials
title_sort Unambiguous detection of atherosclerosis using bioorthogonal nanomaterials
dc.creator.none.fl_str_mv Pellico, Juan
Fernández-Barahona, Irene
Benito, Marina
Gaitán-Simón, Ángel
Gutiérrez, Lucía
Ruiz-Cabello, Jesús
Herranz, Fernando
author Pellico, Juan
author_facet Pellico, Juan
Fernández-Barahona, Irene
Benito, Marina
Gaitán-Simón, Ángel
Gutiérrez, Lucía
Ruiz-Cabello, Jesús
Herranz, Fernando
author_role author
author2 Fernández-Barahona, Irene
Benito, Marina
Gaitán-Simón, Ángel
Gutiérrez, Lucía
Ruiz-Cabello, Jesús
Herranz, Fernando
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Agencia Estatal de Investigación (España)
Ministerio de Economía y Competitividad (España)
Instituto de Salud Carlos III
Ministerio de Ciencia, Innovación y Universidades (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Molecular imaging
Iron oxide
Gallium-68
Atherosclerosis
Nano-radiomaterials
topic Molecular imaging
Iron oxide
Gallium-68
Atherosclerosis
Nano-radiomaterials
description The importance of atherosclerosis is driving research to create improved diagnostic tools based on molecular imaging. Pretargeted imaging is the use of bioorthogonal probes that selectively accumulate upon reaction with a pre-modified biomolecule in vivo. To date, this very promising approach has not been applied to atherosclerosis. Neither has been the use of a single nano-radiomaterial for PET / T-MR imaging of atherosclerosis. Here, we synthesized bioorthogonal nano-radiomaterials for in vivo pretargeted molecular imaging in a mouse model of atherosclerosis. Based on tetrazine-ligation, these functionalized Ga iron oxide nano-radiomaterials provide simultaneous PET and T-MRI signals and selectively accumulate in atherosclerotic plaques in mice sequentially injected with trans-cyclooctene-modified antibodies against oxidized LDL followed by the hybrid nano-radiomaterial. Our results demonstrate the ability of this approach to unambiguously detect atherosclerosis. Furthermore, we show the first example of how hybrid imaging can be used for pretargeted bioorthogonal molecular imaging with nanomaterials.
publishDate 2019
dc.date.none.fl_str_mv 2019
2019
2019
2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/176957
url http://hdl.handle.net/10261/176957
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
SAF2017-84494-C2-R/AEI/10.13039/501100011033
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/SAF2016-79593-P
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/SAF2017-84494-C2-R
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/RYC-2014-15512
http://dx.doi.org/10.1016/j.nano.2018.12.015
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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