Enhanced production of lactococcin 972 in chemostat cultures

Lactococcus lactis subsp. lactis IPLA972 is a wild lactococcal strain suitable as a single starter in the manufacture of dairy products. This strain synthesizes lactococcin 972 (Lcn972), a unique bacteriocin that blocks septum formation. In this work, we report on the conditions to optimize biomass...

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Autores: Hernández de Rojas, Alma, Martínez, B., Suárez, J.E., Rodríguez-de-León, Argeo
Tipo de recurso: artículo
Fecha de publicación:2004
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/327500
Acceso en línea:http://hdl.handle.net/10261/327500
Access Level:acceso abierto
Palabra clave:Centro Oceanográfico de Gijón
Desconocido
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spelling Enhanced production of lactococcin 972 in chemostat culturesHernández de Rojas, AlmaMartínez, B.Suárez, J.E.Rodríguez-de-León, ArgeoCentro Oceanográfico de GijónDesconocidoLactococcus lactis subsp. lactis IPLA972 is a wild lactococcal strain suitable as a single starter in the manufacture of dairy products. This strain synthesizes lactococcin 972 (Lcn972), a unique bacteriocin that blocks septum formation. In this work, we report on the conditions to optimize biomass and Lcn972 production. In batch cultures, pH 6.8 was found to be optimum for bacteriocin synthesis and both glucose and lactose supported Lcn972 production. The inhibitory activity improved up to eight-fold with increasing carbohydrate concentration. In chemostat cultures, steady states were achieved even at dilution rates higher than mu(max), due to the strong wall growth. Lcn972 behaved as a true primary metabolite, as it was maximally produced when the cells were actively growing. Bacteriocin yields were improved up to ten-fold in chemostat cultures compared with those achieved in batch.202320232004info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/327500reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésCentro Oceanográfico de Gijóninfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3275002026-05-22T06:33:51Z
dc.title.none.fl_str_mv Enhanced production of lactococcin 972 in chemostat cultures
title Enhanced production of lactococcin 972 in chemostat cultures
spellingShingle Enhanced production of lactococcin 972 in chemostat cultures
Hernández de Rojas, Alma
Centro Oceanográfico de Gijón
Desconocido
title_short Enhanced production of lactococcin 972 in chemostat cultures
title_full Enhanced production of lactococcin 972 in chemostat cultures
title_fullStr Enhanced production of lactococcin 972 in chemostat cultures
title_full_unstemmed Enhanced production of lactococcin 972 in chemostat cultures
title_sort Enhanced production of lactococcin 972 in chemostat cultures
dc.creator.none.fl_str_mv Hernández de Rojas, Alma
Martínez, B.
Suárez, J.E.
Rodríguez-de-León, Argeo
author Hernández de Rojas, Alma
author_facet Hernández de Rojas, Alma
Martínez, B.
Suárez, J.E.
Rodríguez-de-León, Argeo
author_role author
author2 Martínez, B.
Suárez, J.E.
Rodríguez-de-León, Argeo
author2_role author
author
author
dc.subject.none.fl_str_mv Centro Oceanográfico de Gijón
Desconocido
topic Centro Oceanográfico de Gijón
Desconocido
description Lactococcus lactis subsp. lactis IPLA972 is a wild lactococcal strain suitable as a single starter in the manufacture of dairy products. This strain synthesizes lactococcin 972 (Lcn972), a unique bacteriocin that blocks septum formation. In this work, we report on the conditions to optimize biomass and Lcn972 production. In batch cultures, pH 6.8 was found to be optimum for bacteriocin synthesis and both glucose and lactose supported Lcn972 production. The inhibitory activity improved up to eight-fold with increasing carbohydrate concentration. In chemostat cultures, steady states were achieved even at dilution rates higher than mu(max), due to the strong wall growth. Lcn972 behaved as a true primary metabolite, as it was maximally produced when the cells were actively growing. Bacteriocin yields were improved up to ten-fold in chemostat cultures compared with those achieved in batch.
publishDate 2004
dc.date.none.fl_str_mv 2004
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/327500
url http://hdl.handle.net/10261/327500
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Centro Oceanográfico de Gijón
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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