Transcriptomic profile of epileptic children treated with ketogenic therapies.

Background: Ketogenic dietary therapies (KDT) are used as a treatment in childhood epilepsy. However, their mechanism has not yet been established. The main objective of this study was to determine the changes in the transcriptomic profile induced by KDT in children with epilepsy in order to shed li...

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Autores: Ruiz-Herrero, Jana, Olaso-Gonzalez, Gloria, Serna, Eva, Canedo-Villarroya, Elvira, Correas, Angela G, Gambini, Juan, Gomez-Cabrera, Mari Carmen, Pedron-Giner, Consuelo, Vina, Jose
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:España
Institución:INCLIVA
Repositorio:r-INCLIVA. Repositorio Institucional de Producción Científica de INCLIVA
OAI Identifier:oai:incliva.fundanetsuite.com:p16435
Acceso en línea:https://incliva.portalinvestigacion.com/publicaciones/16435
Access Level:acceso abierto
Palabra clave:Anticonvulsant
Epilepsy
Ketogenic diet
Ketogenic dietary therapies
Synapsis
Transcriptome
miRNome
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repository_id_str
spelling Transcriptomic profile of epileptic children treated with ketogenic therapies.Ruiz-Herrero, JanaOlaso-Gonzalez, GloriaSerna, EvaCanedo-Villarroya, ElviraCorreas, Angela GGambini, JuanGomez-Cabrera, Mari CarmenPedron-Giner, ConsueloVina, JoseAnticonvulsantEpilepsyKetogenic dietKetogenic dietary therapiesSynapsisTranscriptomemiRNomeBackground: Ketogenic dietary therapies (KDT) are used as a treatment in childhood epilepsy. However, their mechanism has not yet been established. The main objective of this study was to determine the changes in the transcriptomic profile induced by KDT in children with epilepsy in order to shed light on its possible mechanisms. Methods: Eight children with refractory epilepsy were enrolled in the study. Peripheral blood mononuclear cells were obtained before and after the children were treated with KDT for a minimum of 6 months. RNA was extracted and mRNA and miRNA profiling were performed and analyzed. Results: Our intervention with KDT significantly reduced the seizure number in seven of the eight paediatric patients treated and caused important changes in their gene expression profile. Our study reveals modifications in the transcription of 4630 genes and 230 miRNAs. We found that the genes involved in the protection against epileptic crises were among those mainly changed. These genes collectively encode for ion channels, neurotransmitter receptors, and synapse structural proteins. Conclusions: Together our results explain the possible mechanisms of KDT and reinforce its clinical importance in the treatment of epilepsy.IMR PRESS2022info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://incliva.portalinvestigacion.com/publicaciones/16435Journal of Integrative NeuroscienceISSN: 02196352ISSNe: 1757448Xreponame:r-INCLIVA. Repositorio Institucional de Producción Científica de INCLIVAinstname:INCLIVAInglésinfo:eu-repo/semantics/openAccessoai:incliva.fundanetsuite.com:p164352026-06-07T16:35:31Z
dc.title.none.fl_str_mv Transcriptomic profile of epileptic children treated with ketogenic therapies.
title Transcriptomic profile of epileptic children treated with ketogenic therapies.
spellingShingle Transcriptomic profile of epileptic children treated with ketogenic therapies.
Ruiz-Herrero, Jana
Anticonvulsant
Epilepsy
Ketogenic diet
Ketogenic dietary therapies
Synapsis
Transcriptome
miRNome
title_short Transcriptomic profile of epileptic children treated with ketogenic therapies.
title_full Transcriptomic profile of epileptic children treated with ketogenic therapies.
title_fullStr Transcriptomic profile of epileptic children treated with ketogenic therapies.
title_full_unstemmed Transcriptomic profile of epileptic children treated with ketogenic therapies.
title_sort Transcriptomic profile of epileptic children treated with ketogenic therapies.
dc.creator.none.fl_str_mv Ruiz-Herrero, Jana
Olaso-Gonzalez, Gloria
Serna, Eva
Canedo-Villarroya, Elvira
Correas, Angela G
Gambini, Juan
Gomez-Cabrera, Mari Carmen
Pedron-Giner, Consuelo
Vina, Jose
author Ruiz-Herrero, Jana
author_facet Ruiz-Herrero, Jana
Olaso-Gonzalez, Gloria
Serna, Eva
Canedo-Villarroya, Elvira
Correas, Angela G
Gambini, Juan
Gomez-Cabrera, Mari Carmen
Pedron-Giner, Consuelo
Vina, Jose
author_role author
author2 Olaso-Gonzalez, Gloria
Serna, Eva
Canedo-Villarroya, Elvira
Correas, Angela G
Gambini, Juan
Gomez-Cabrera, Mari Carmen
Pedron-Giner, Consuelo
Vina, Jose
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Anticonvulsant
Epilepsy
Ketogenic diet
Ketogenic dietary therapies
Synapsis
Transcriptome
miRNome
topic Anticonvulsant
Epilepsy
Ketogenic diet
Ketogenic dietary therapies
Synapsis
Transcriptome
miRNome
description Background: Ketogenic dietary therapies (KDT) are used as a treatment in childhood epilepsy. However, their mechanism has not yet been established. The main objective of this study was to determine the changes in the transcriptomic profile induced by KDT in children with epilepsy in order to shed light on its possible mechanisms. Methods: Eight children with refractory epilepsy were enrolled in the study. Peripheral blood mononuclear cells were obtained before and after the children were treated with KDT for a minimum of 6 months. RNA was extracted and mRNA and miRNA profiling were performed and analyzed. Results: Our intervention with KDT significantly reduced the seizure number in seven of the eight paediatric patients treated and caused important changes in their gene expression profile. Our study reveals modifications in the transcription of 4630 genes and 230 miRNAs. We found that the genes involved in the protection against epileptic crises were among those mainly changed. These genes collectively encode for ion channels, neurotransmitter receptors, and synapse structural proteins. Conclusions: Together our results explain the possible mechanisms of KDT and reinforce its clinical importance in the treatment of epilepsy.
publishDate 2022
dc.date.none.fl_str_mv 2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://incliva.portalinvestigacion.com/publicaciones/16435
url https://incliva.portalinvestigacion.com/publicaciones/16435
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv IMR PRESS
publisher.none.fl_str_mv IMR PRESS
dc.source.none.fl_str_mv Journal of Integrative Neuroscience
ISSN: 02196352
ISSNe: 1757448X
reponame:r-INCLIVA. Repositorio Institucional de Producción Científica de INCLIVA
instname:INCLIVA
instname_str INCLIVA
reponame_str r-INCLIVA. Repositorio Institucional de Producción Científica de INCLIVA
collection r-INCLIVA. Repositorio Institucional de Producción Científica de INCLIVA
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.812429

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